As stated in the 'PROPERTIES' section, each vial contains 1 mL. One ampule contains 1 mL of Dimethyl Sulfoxide, and 5 amps include 5 ampules containing 1 mL of Dimethyl Sulfoxide. each.
D9170
Diméthylsulfoxyde
PCR Reagent
Synonyme(s) :
DMSO, Sulfoxyde de méthyle
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1 AMP
36,20 €
5 AMP
132,00 €
36,20 €
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A propos de cet article
Formule linéaire :
(CH3)2SO
Numéro CAS:
Poids moléculaire :
78.13
UNSPSC Code:
12191502
NACRES:
NA.25
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Assay:
≥99.5%
Technique(s):
PCR: suitable
Application(s):
agriculture
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)
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Laissez-nous vous aiderNom du produit
Diméthylsulfoxyde, PCR Reagent
InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
SMILES string
CS(C)=O
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
assay
≥99.5%
form
liquid
autoignition temp.
573 °F
shelf life
Recommended retest period - 2 years
expl. lim.
42 %, 63 °F
packaging
vial of 1 mL
technique(s)
PCR: suitable
impurities
≤0.1% water
water
color
colorless
refractive index
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: miscible (completely)
density
1.10 g/mL (lit.)
suitability
suitable for PCR
application(s)
agriculture
Quality Level
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Catégories apparentées
1 of 4
Cet article | 34869 | 41640-M | D8418 |
|---|---|---|---|
| application(s) agriculture | application(s) food and beverages | application(s) - | application(s) - |
| grade PCR Reagent | grade - | grade ACS reagent, puriss. p.a. | grade for molecular biology |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 200 |
| assay ≥99.5% | assay ≥99.7% | assay ≥99.9% (GC) | assay ≥99.9% |
| technique(s) PCR: suitable | technique(s) HPLC: suitable | technique(s) - | technique(s) DNA sequencing: suitable, PCR: suitable, transfection: suitable |
| solubility H2O: miscible (completely) | solubility - | solubility - | solubility H2O: miscible (completely) |
Disclaimer
Refroidit facilement et revient doucement à la température de la pièce. Les produits solides peuvent être reliquéfiés par chauffage à la température ambiante sans abîmer le produit.
Application
General description
Dimethyl sulfoxide is a free radical scavenger and a cryopreservant. It elicits protective effect in double stranded breaks(DSBs).[4] Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination.[2] As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.[5]
Other Notes
For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table
Dimethyl Sulfoxide Miscibility/Immiscibility Table
Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials
Ravikumar et al.
Applied Biochemistry and Biotechnology, 175(1), 3856-3862 (2015)
A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and gamma-ray-irradiation, and freezing
Noda M, et al.
Scientific reports, 7(1), 8557-8557 (2017)
Microtitre Plate Based Cell-SELEX Method
Shorie M and Kaur H
Bio-protocol, 8(20) (2018)
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
Lorenz, Todd C
Journal of Visualized Experiments, (63) (2012)
IRAP and REMAP for retrotransposon-based genotyping and fingerprinting
Kalendar R and Schulman AH
Nature Protocols, 1(5), 2478-2478 (2006)
Contenu apparenté
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How much DMSO is in each of the vials (D9170)?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Where does DMSO come from?
1 answer-
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
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Dimethyl sulfoxide (DMSO) should be a liquid. Why did it arrive as a solid?
1 answer-
DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.
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Does DMSO have any synthetic utility?
1 answer-
DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molarity of DMSO?
1 answer-
DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
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What is the DMSO dissolved in?
1 answer-
DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.
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