FCANOS1
Nitric Oxide Synthase Detection System, Fluorimetric
sufficient for 200 reactions
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752,00 €
752,00 €
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A propos de cet article
Code UNSPSC :
12352303
Nomenclature NACRES :
NA.32
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Laissez-nous vous aiderUtilisation
sufficient for 200 reactions
Niveau de qualité
Disponibilité
not available in Japan
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Informations sur le gène
human ... NOS1(4842), NOS2(4843), NOS2B(201288), NOS2C(645740), NOS3(4846)
1 of 4
Cet article | N2783 | SAB4200766 | 482655 |
|---|---|---|---|
| Gene Information human ... NOS1(4842), NOS2(4843), NOS2B(201288), NOS2C(645740), NOS3(4846) | Gene Information mouse ... Nos2(18126) | Gene Information mouse ... Nos2(18126) | Gene Information - |
| usage sufficient for 200 reactions | usage - | usage - | usage sufficient for 192-288 tests |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in - |
| storage temp. 2-8°C | storage temp. −70°C | storage temp. −20°C | storage temp. −20°C |
| availability not available in Japan | availability - | availability - | availability - |
Actions biochimiques/physiologiques
Simple and specific assay for the measurement of free nitric oxide (NO) and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Nitric oxide, formed from the amino acid L-arginine via the action of NOS, is a unique cell signaling molecule that functions as both an intracellular and an extracellular messenger. NO has been implicated in vasodilation, neurotransmission, cytotoxicity, and inflammation.
The standard assay for NOS activity is for solubilized cell-free enzyme preparations such as cell lysates, crude tissue extracts or purified enzymes. It measures the production of radiolabeled citrulline from radiolabeled arginine substrate (Sigma Product No. NOS-1). In contrast, the Fluorometric Cell-Associated NOS Detection System measures intracellular production of NO by a non-radiometric method. Radioisotopes are replaced by a cell-permeable diacetate derivative of 4,5-diaminofluorescein (DAF-2 DA). DAF-2 DA penetrates cells rapidly where it is hydrolyzed by intracellular esterase to DAF-2 that, in turn, reacts with NO produced by NOS to form a fluorescent triazolo-fluorescein. The fluorescent product can be quantitated using an excitation filter at 492 nm and an emission filter at 515 nm.
The standard assay for NOS activity is for solubilized cell-free enzyme preparations such as cell lysates, crude tissue extracts or purified enzymes. It measures the production of radiolabeled citrulline from radiolabeled arginine substrate (Sigma Product No. NOS-1). In contrast, the Fluorometric Cell-Associated NOS Detection System measures intracellular production of NO by a non-radiometric method. Radioisotopes are replaced by a cell-permeable diacetate derivative of 4,5-diaminofluorescein (DAF-2 DA). DAF-2 DA penetrates cells rapidly where it is hydrolyzed by intracellular esterase to DAF-2 that, in turn, reacts with NO produced by NOS to form a fluorescent triazolo-fluorescein. The fluorescent product can be quantitated using an excitation filter at 492 nm and an emission filter at 515 nm.
Informations légales
DAF-2 DA is manufactured by Daiichi Pure Chemicals Co., LTD.
US Patent No. 5,874, 590
US Patent No. 5,874, 590
Composants de kit seuls
Réf. du produit
Description
- Diphenyleneiodonium Chloride (DPI) 1 mM 200 μL
- Arginine Substrate Solution for NOS Detection, Fluorimetric 2 mL
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Sonia Agrawal et al.
Scientific reports, 11(1), 19813-19813 (2021-10-08)
The development of the latent phenotype of Mycobacterium tuberculosis (Mtb) in the human lungs is the major hurdle to eradicate Tuberculosis. We recently reported that exposure to nitrite (10 mM) for six days under in vitro aerobic conditions completely transforms the
Zeming Zhang et al.
Journal of cellular physiology, 234(11), 19715-19727 (2019-04-16)
Pulmonary arterial hypertension (PAH) is characterized by pulmonary vascular remodeling of the precapillary pulmonary arteries, with excessive proliferation of vascular cells. This study was performed to examine the effects of long noncoding RNA CPS1 intronic transcript 1 (CPS1-IT) on PAH
D A Geller et al.
Cancer metastasis reviews, 17(1), 7-23 (1998-04-17)
Nitric oxide (NO) is a potent biologic mediator with diverse physiologic and pathophysiologic roles. NO is produced from L-arginine by the family of nitric oxide synthase (NOS) enzymes, forming the free radical NO and citrulline as byproduct. Three distinct isoforms
D J Stuehr
Annual review of pharmacology and toxicology, 37, 339-359 (1997-01-01)
Research on the biological roles of nitric oxide has revealed that it functions as an important signal and effector molecule in a variety of physiologic and pathologic settings. In animals, nitric oxide is synthesized enzymatically from L-arginine through the actions
D S Bredt et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(22), 9030-9033 (1989-11-01)
Nitric oxide, which mediates influences of numerous neurotransmitters and modulators on vascular smooth muscle and leukocytes, can be formed in the brain from arginine by an enzymatic activity that stoichiometrically generates citrulline. We show that glutamate and related amino acids
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