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Merck

H9268

Hexadimethrine bromide

≥94% (titration)

Synonyme(s) :

1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide, Polybrene

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H9268-5G

72,40 €

H9268-10G

128,00 €

H9268-50G

435,00 €

H9268-100G

719,00 €

72,40 €


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A propos de cet article

Numéro CAS:
Numéro MDL:
Code UNSPSC :
41106502
Nomenclature NACRES :
NA.26

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Niveau de qualité

Essai

≥94% (titration)

Température de stockage

2-8°C

Chaîne SMILES 

BrCCCBr.N(CCCCCCN(C)C)(C)C

InChI

1S/C10H24N2.C3H6Br2/c1-11(2)9-7-5-6-8-10-12(3)4;4-2-1-3-5/h5-10H2,1-4H3;1-3H2

Clé InChI

KZKAYEGOIJEWQB-UHFFFAOYSA-N

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Cet article
S2667107689TR-1003
storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

assay

≥94% (titration)

assay

-

assay

≥95%

assay

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

Description générale

Hexadimethrine bromide is a quaternary ammonium compound which works as a heparin antagonist.[1] It is also involved in the transformation of low molecular weight DNA.[2]

Application

Hexadimethrine bromide can be used to transfect mammalian cells with DNA. It can be used to increase the efficiency of lipofection transfections.
Hexadimethrine bromide has been used for lentivirus infection in cells.[3][4] It has been used for infection of cells with retroviral supernatant.[5][6]

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Acute Tox. 4 Oral

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Redei GP
Encyclopedia of Genetics, Genomics, Proteomics, and Informatics (2008)
Blood (2013)
UCHL1 Is a Putative Tumor Suppressor in Ovarian Cancer Cells and Contributes to Cisplatin Resistance.
Jin C et al.
Journal of Cancer, 4, 662-662 (2013)
Molecular pathways regulating pro-migratory effects of Hedgehog signaling.
Hochman E et al.
The Journal of Biological Chemistry, 281, 33860-33860 (2006)
Gaetana Restivo et al.
Nature communications, 8(1), 1988-1988 (2017-12-08)
Cutaneous melanoma represents the most fatal skin cancer due to its high metastatic capacity. According to the "phenotype switching" model, the aggressive nature of melanoma cells results from their intrinsic potential to dynamically switch from a high-proliferative/low-invasive to a low-proliferative/high-invasive

Articles

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Protocoles

Detailliertes Verfahren zur Durchführung einer lentiviralen Transduktion von MISSION shRNA lentiviralen Partikeln, um eine stabile, langfristige Stilllegung und Phänotypveränderung zu erreichen.

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Questions

1–10 of 12 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Is it possible for hexadimethrine bromide to breakdown and lose its effectiveness after 3 years when stored in its powdered form at 4 degree celsius? We bought this product around 3 years and now it doesn't work as well as before when doing transduction.

    1 answer
    1. This product is assigned a recommended retest period of 2 years from the date of Quality release. While quite stable, the compound may slowly degrade over time. The suitability of this item beyond the lot specific retest date would have to be determined by the end user.

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  4. what is the concentration of 5G polybrene? Does G stands for gram?

    1 answer
    1. This product is in powder form and not a prepared solution. The minimum purity of this material is 94%. The 'G' represents grams.

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  5. What is the preferred solvent for Hexadimethrine bromide? I see water or saline above, but does either effect solubility or stability?

    1 answer
    1. The choice of solvent typically depends on the specific application. For in vitro or in vivo studies, saline is more commonly used. The material is soluble in water at up to 10%. Solutions are quite stable and maintain activity after autoclaving. Stock solutions prepared at 1 mg/mL in saline are stable for up to 1 year when stored at 2–8 °C. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/253/063/h9268bul.pdf

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  6. How long is the Hexadimethrine Bromide powder stable? I cannot find its expiry date.

    1 answer
    1. This product in powdered format is stable for up to a recommended retest period of two years as listed on the Certificate of Analysis. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      When in solution, this product will remain active for at least one year if stored at 2–8 °C. For more information please view the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/253/063/h9268bul.pdf

      Helpful?

  7. What is the solution stability of Product H9268, Hexadimethrine bromide?

    1 answer
    1. Stock solutions in 0.9% saline solution at 1 mg/mL are stable at least one year stored in the refrigerator.  Stability information is found on the Product Bulletin.

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  8. How do I sterilize Product H9268, Hexadimethrine bromide?

    1 answer
    1. Hexadimethrine bromide can be filtered sterilized or autoclaved.

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  9. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. What is the molecular weight of Product H9268, Hexadimethrine bromide?

    1 answer
    1. According to the information from our supplier, the molecular weight of this product is 4,000-6,000.The chemicals encyclopedia published by the Royal Society of Chemistry provides a general MW range for the chemical of 5,000 - 10,000.

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1–10 of 12 Questions  

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