The suggested coating protocol involves removing excess solution by aspiration and rinsing the vessel with sterile water. Collecting residual poly-L-ornithine (PLO) solution and storing it at 4 °C for reuse has not been tested internally. External sources indicate that the PLO solution can be reused after coating, and this practice is common in many laboratories. When reusing the PLO solution, it is essential to utilize a sterile hood and aseptic techniques to prevent contamination.
P4957
Poly-ʟ-Ornithine
synthetic, mol wt 30,000-70,000, liquid, 0.01%, suitable for cell culture
Poly-L-ornithine solution
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50 ML
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A propos de cet article
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352202
ID de substance PubChem :
Nomenclature NACRES :
NA.75
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Poly-L-ornithine solution, mol wt 30,000-70,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture
Source biologique
synthetic
Stérilité
sterile-filtered
Gamme de produits
BioReagent
Forme
solution
Poids mol.
30,000-70,000
Conditionnement
pkg of 50 mL
Concentration
0.01%
Technique(s)
cell culture | mammalian: suitable
Couverture de surface
4 μg/cm2
Impuretés
endotoxin, tested
Conditions d'expédition
ambient
Température de stockage
2-8°C
Chaîne SMILES
Br.NCCCC(N)C(O)=O
InChI
1S/C5H12N2O2.BrH/c6-3-1-2-4(7)5(8)9;/h4H,1-3,6-7H2,(H,8,9);1H
Clé InChI
GWRQMKDBBHFVIZ-UHFFFAOYSA-N
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Cet article | P4707 | O6503 | P5899 |
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility γ-irradiated |
| biological source synthetic | biological source synthetic | biological source microbial | biological source synthetic (organic) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| concentration 0.01% | concentration 0.01% | concentration - | concentration - |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| mol wt 30,000-70,000 | mol wt 70—150 kDa | mol wt - | mol wt ≥300,000 |
Description générale
La poly-L-ornithine est un polymère de synthèse d′un acide aminé chargé positivement.[1]
Application
La poly-L-ornithine a été utilisée avec succès pour l′attachement de cellules neuronales et pour étudier leur différenciation et excroissance. Comme substrat de culture cellulaire, il est recommandé d′utiliser 0,5 ml de solution à 0,1 mg/ml pour recouvrit 25 cm2.
La solution de poly-L-ornithine a été employée pour induire la différenciation.[2]
Actions biochimiques/physiologiques
Notes préparatoires
Préparer dans de l′eau de qualité culture cellulaire.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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will it be effective to reuse poly-L-ornithine (PLO), either in diluted or undiluted form? Following a coating period of 1 hour or overnight, can the residual PLO solution be collected, stored at 4 °C, and subsequently reused for additional coatings?
1 answer-
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ho ricevuto questo prodotto a temperatura ambiente, anche se lo storage consigliato é 4°C. E' da ritenersi ugualmente efficace?
1 answer-
This item is stored refrigerated at 2-8°C. However, the product is shipped at ambient temperature. Shipping routes are configured to minimize transit time.
Refrigerated products that ship at ambient temperature are considered stable up to 2 weeks for transit time.Helpful?
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CAt#P4957 do I just coat with the stock solution? at what conditions? RT or 37C or other? For how long?
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This product is a 0.01% or 100 ug/mL solution. The recommended surface coverage for this product is 4 μg/cm2. Provided the surface area of the vessel accommodates this concentration, the product may be used undiluted. A T-25 Tissue Culture flask has a surface area of 25 cm2.
In this case. 1 mL of the undiluted product would be sufficient to coat the entire culture surface. Conversely, a 96 well flat bottom plate will have a culture area of approximately 0.150cm2 requiring approximately 0.6 ug to coat the surface. In this example, it may be more practical to dilute to a practical volume. Surfaces may be coated at room temperature. Incubate at least 5 minutes before removing excess solution. Coated vessels may be sterilized by UV and stored in a sealed container for up to 1 year. Avoid using coated vessels or slides that have any yellowing or changes in the surface appearance as this is an indication of degradation.
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Will poly-L-ornithine still be effective if it is incubated for 2 hours at 37°C, as done with other substrates, instead of being coated at room temperature overnight as indicated?
1 answer-
The optimal coating conditions, including the required incubation time and temperature, are application-dependent and should be determined empirically. For reference, this product has been reported to coat well at 37°C with just 30 minutes of incubation time in a publication that may be of interest (Nature. 2019 Aug;572(7770):543-548. doi: 10.1038/s41586-019-1464-0. Epub 2019 Aug 7. Pol II phosphorylation regulates a switch between transcriptional and splicing condensates, PMID: 31391587).
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