SAE0088
Filipin complex ready made solution
from Streptomyces filipinensis, 5mg/ml in DMSO based solution
Synonyme(s) :
Filipin solution
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1 ML
308,00 €
308,00 €
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A propos de cet article
NACRES:
NA.77
UNSPSC Code:
12352200
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Filipin complex ready made solution, from Streptomyces filipinensis, 5mg/ml in DMSO based solution
assay
≥90% (HPLC)
form
ready-to-use solution
storage condition
protect from light
shipped in
dry ice
storage temp.
−20°C
Catégories apparentées
1 of 4
Cet article | SAE0087 | F9765 | SML0907 |
|---|---|---|---|
| form ready-to-use solution | form ready-to-use solution | form powder | form DMSO solution |
| assay ≥90% (HPLC) | assay ≥95% (HPLC) | assay ≥70% (UV) | assay - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in dry ice |
| storage condition protect from light | storage condition protect from light | storage condition - | storage condition - |
Application
Filipin complex readymade solution has been used in the staining of HeLa cells for cytoskeletal imaging.[1]
Filipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.
Biochem/physiol Actions
Filipin is an antibiotic with antifungal functionality.[2] Filipin complex has been found to alter cell membrane structure by interacting with the membrane sterols ergosterols and cholesterol.[3] On binding to cholesterol, filipin shows fluorescence shift. As such, filipin complex has become a useful tool for diagnosis of Niemann-Pick type C disease, and for detection and quantification of cholesterol in cell membranes and lipid quantification in brain.[2]
General description
Filipin Complex, the collective name given to family of isomeric polyene macrolides isolated from cultures of S. filipinensis, with Filipin III being the majority part of the complex.[3]
Other Notes
Filipin complex ready made solution is very sensitive to air and light. Upon receipt aliquot and store at -20°C. Avoid freeze thaw cycles.
Filipin complex is used in various concentrations depending on the specific protocol used. Dilute 5mg/ml stock solution with appropriate buffer according to the used protocol.
Filipin complex interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).
Filipin complex is used in various concentrations depending on the specific protocol used. Dilute 5mg/ml stock solution with appropriate buffer according to the used protocol.
Filipin complex interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).
Classe de stockage
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Analysis of cholesterol trafficking with fluorescent probes
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