SRP0100

HDAC-1 human

recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)

• Synonyme(s) : HD1, Histone deacetylase 1
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Essai: ≥50% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 56 kDa
• Conditionnement: pkg of 50 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_004964
• Numéro d'accès UniProt: Q13547
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... HDAC1(3065)

Description

Description générale

Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal HIS-DDDDK tag (FLAG) and C-terminal His-tag, MW = 56 kDa, expressed in baculovirus expression system.

Actions biochimiques/physiologiques

Useful for the study of enzyme kinetics, screening inhibitors.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Autres remarques

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable