This product is prepared in DMSO at a concentration of 3.125 mg/mL, which equates to approximately 5 mM. The volume of the 1 mg package is 0.320 mL. Prior to use, dilute the product 500-fold in 0.1 M phosphate buffer, pH 7.4. Other aqueous buffers such as HBSS would also be suitable.
Note that the presence of BSA and phenol red may interfere with the fluorescence.
251765
DAR-4M AM
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.
Synonym(s):
DAR-4M AM, Diaminorhodamine-4M AM
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About This Item
Empirical Formula (Hill Notation):
C28H31IN4O5
Molecular Weight:
630.47
NACRES:
NA.25
UNSPSC Code:
12352202
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Product Name
DAR-4M AM, A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.
assay
≥98% (HPLC)
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
dark red
fluorescence
λex ~560 nm
λem ~575 nm
shipped in
ambient
storage temp.
−20°C
Quality Level
Related Categories
1 of 4
This Item | 251760 | 251520-M | 343242-M |
|---|---|---|---|
| fluorescence λex ~560 nm, λem ~575 nm | fluorescence λex ~560 nm, λem ~575 nm | fluorescence λex ~500 nm, λem ~515 nm | fluorescence - |
| color dark red | color red-violet | color pale yellow | color red-orange |
| form solution | form solution | form solution | form solid |
| shipped in ambient | shipped in ambient | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Nitric oxide (NO) fluorescent indicator
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no
Disclaimer
Toxicity: Irritant (B)
General description
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.
Legal Information
Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Packaging
Packaged under inert gas
Physical form
In DMSO.
Preparation Note
Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F
flash_point_c
87 °C
Certificates of Analysis (COA)
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The product is provided in solution form but no information is provided about its volume. Please provide the volume and molarity of the solution. Also what would be the recommended way of dilution - in DMSO or directly in HBSS/PBS/media?
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