The optimal recommended units of chitinase per milliliter (U/mL) for fungal DNA extraction vary depending on the protocol and fungal species employed. For protocol and working concentration reference, a relevant citation is available in Fungal Biology and Biotechnology 12.1 (2025): 1-17.
C6137
Chitinase from Streptomyces griseus
lyophilized powder (essentially salt free), ≥200 units/g solid
Synonym(s):
N-acetyl-glucosaminidasechitobiase, Chitin digestion enzymes, poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase
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5 UNITS
R$821.00
25 UNITS
R$2,357.00
50 UNITS
R$4,058.00
R$821.00
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About This Item
UNSPSC Code:
12352204
EC Number:
232-578-7
NACRES:
NA.54
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Product Name
Chitinase from Streptomyces griseus, lyophilized powder (essentially salt free), ≥200 units/g solid
form
lyophilized powder (essentially salt free)
specific activity
≥200 units/g solid
mol wt
30 kDa
solubility
H2O: soluble 0.90-1.10 mg/mL
storage temp.
−20°C
Quality Level
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Related Categories
1 of 4
This Item | C8241 | SAE0158 | C9830 |
|---|---|---|---|
| specific activity ≥200 units/g solid | specific activity ≥600 units/g solid | specific activity >200 USP units/mg solid | specific activity >50 units/mg protein (Bradford) |
| form lyophilized powder (essentially salt free) | form lyophilized powder | form - | form lyophilized powder |
| solubility H2O: soluble 0.90-1.10 mg/mL | solubility 0.05 M phosphate buffer, pH 6.0: soluble 0.90-1.10 mg/mL, faintly hazy to hazy (with particles) | solubility - | solubility H2O: soluble, clear to slightly hazy, colorless (10U/0.3mL) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 30 kDa | mol wt 30 kDa | mol wt - | mol wt - |
| Quality Level 300 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
Application
Agriculture fields: control pathogens.
Human health care: Asthma.
Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,
Preparation of single-cell protein
Isolation of protoplasts from fungi and yeast
Control of pathogenic fungi
Treatment of chitinous waste, mosquito control and morphogenesis
Human health care: Asthma.
Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,
Preparation of single-cell protein
Isolation of protoplasts from fungi and yeast
Control of pathogenic fungi
Treatment of chitinous waste, mosquito control and morphogenesis
Biochem/physiol Actions
Chitinase is an extracellular enzyme complex that degrades chitin and has a molecular mass of approximately 30 kDa. Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase. The second reaction involves N-acetyl-glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits (that comprise of N-acetyl-D-glucosamine). The optimum reaction temperature is 37 °C.
Features and Benefits
Chitinase is an extracellular complex of enzymes that degrade chitin. It is a lytic enzyme suitable for fungal cell walls lysis.
General description
Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeketal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates) . Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source.Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source.
The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:
The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:
- The first reaction, chitodextrinase-chitinase, is a poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, which removes chitobiose units from chitin.
- The second activity is N-acetyl-glucosaminidasechitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine.
Other Notes
One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C in a 2 hour assay.
One new 1 hour unit = approx. 50 old 48 hour units.
One new 1 hour unit = approx. 50 old 48 hour units.
used together
Product No.
Description
Pricing
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Shigeo Suzuki et al.
International journal of biological macromolecules, 43(1), 13-19 (2007-11-06)
Allosamidin is a family 18 chitinase inhibitor produced by Streptomyces. In its producing strain, Streptomyces sp. AJ9463, allosamidin promotes production of the family 18 chitinase originated from chi65 in a chitin medium through the two-component regulatory system encoded by chi65R
Melissa S Hillwig et al.
Journal of plant physiology, 168(7), 734-738 (2010-11-26)
Previous SDS PAGE gel analysis of the floral nectars from petunia and tobacco plants revealed significant differences in the protein patterns. Petunia floral nectar was shown to contain a number of RNase activities by in gel RNase activity assay. To
Xinyu Liu et al.
Bio-protocol, 7(5), e2164-e2164 (2017-03-05)
Chitin is a linear polysaccharide composed of β (1→4)-linked N-acetylglucosamine (GlcNAc) residues. In fungi, chitin is an important component of the cell wall. Here, we provide a protocol to measure the chitin content of fungal cells using Magnaporthe oryzae as
Chitinase-resistant hydrophilic symbiotic factors secreted by Frankia activate both Ca(2+) spiking and NIN gene expression in the actinorhizal plant Casuarina glauca.
Chabaud, et al.
The New phytologist, 209, 86-93 (2020)
S Machida et al.
The Journal of biological chemistry, 268(3), 1702-1707 (1993-01-25)
The membrane-bound chitin synthase, a key enzyme of chitin biosynthesis, was purified, for the first time to homogeneity as a zymogen form. Digitonin could solubilize the enzyme from microsomal fraction of the filamentous fungus Absidia glauca, with 60-70% of the
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How many U/ml of Chitinase are recommended to use for fungal DNA extraction?
1 answer-
Helpful?
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How many grams of chitinase contain the 5 units product CG137-5UN? How much water i need to use to prepare the solution with standard concentration of the enzyme? Kind regards,
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The Chitinase is provided based on units of activity, and the protein mass will vary for individual lots. The specification states that the specific activity will be ≥ 50.00 units per mg protein. The specification sheet can be found here:
https://www.sigmaaldrich.com/specification-sheets/176/740/C9830-10UN________SIGMA____.pdfThe Data Sheet provides the following reconstitution instructions:
Prepare a stock solution by reconstituting a 10 unit vial with 300 ul of water. For long term solution storage reconstitute vial with 50% glycerol solution.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/285/698/c9830dat.pdfHelpful?
-
The Chitinase is provided based on units of activity, and the protein mass will vary for individual lots. The specification states that the specific activity will be ≥ 50.00 units per mg protein. The specification sheet can be found here:
https://www.sigmaaldrich.com/specification-sheets/176/740/C9830-10UN________SIGMA____.pdfThe Data Sheet provides the following reconstitution instructions:
Prepare a stock solution by reconstituting a 10 unit vial with 300 ul of water. For long term solution storage reconstitute the vial with 50% glycerol solution.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/285/698/c9830dat.pdfHelpful?
-
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