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Cell Culture & Cell Culture Analysis Learning Hub

Introduction

Cell culture is an in vitro technique that provides an environment to study cell behavior, including development and differentiation. Using cell culture, researchers can investigate and use cells as models for basic life science research, personalized medicine, and drug discovery, including vaccine manufacturing and therapeutic antibody development.

Cell culture encompasses techniques from two-dimensional to three-dimensional cultures and involves mammalian cells, primary and stem cells, organoids, spheroids, and more. These cells require an environment that includes specific media, nutrients, and sera, and a sterile environment to prevent contamination. Learn more about the different types of cell culture, the reagents and supplements involved in culture, the cell-based assays researchers can perform, and the tools used to gain information about cellular function and behavior.

Section Overview

Introduction

Featured Articles

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Scepter™ 3.0 Handheld Cell Counter

Scepter™ Cell Counter is a portable device which brings consistency in cell counting right to the culture hood in less than 30 seconds.

Lab worker looking at a bottle of fluid on a shelf.

Cell Culture Contamination Troubleshooting

Common Cell Culture Problems: Contamination is easily the most common problem encountered in cell culture laboratories, sometimes with very serious consequence.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Patient-Derived Organoid Biobanks for Drug Discovery

Explore HUB patient-derived organoid biobanks and screening platforms for personalized medicine and drug discovery.

3D Organoid Culture: New In Vitro Models of Development and Disease

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Cell Culture & Cell Culture Analysis Techniques & Technologies

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Cell Analysis

An overview of cellular analysis methods including detailed protocols, technical notes, and videos covering cell counting and cytometry, cell imaging techniques, and cell-based assays.

Cell Culture by Technique

Common cell culture and tissue culture techniques including media preparation, aseptic technique, cell growth and maintenance, and transfection and gene editing.

Cell Culture by Cell Type

Methods for tissue culture support basic and clinical research, drug discovery, and agricultural development.

Cell Culture Troubleshooting

Expert tips for correcting common problems with cell culture including bacterial and fungal contamination, debris in culture media, cell detachment or clumping, slow growth, and low viability.