14-750-M

Abl (H396P) Protein, active, 10 µg

Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the H396P mutation. Expressed by baculovirus in Sf21 insect cells. For use in Kinase Assays.

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 300
• recombinant: expressed in baculovirus infected Sf21 cells
• mol wt: M_w 121 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• UniProt accession no.: P00519; U07563

Description

General description

Product Source: N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the H396P mutation. Expressed by baculovirus in Sf21 insect cells.

The Abl tyrosine kinase inhibitor STI571 (Gleevec) is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse, due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the H396P mutation, which confers resistance to drug binding due to steric interference. (Corbin AS. et al, Blood, (2002); 101:4611-4614 and La Rosee P. et al, Cancer Research; 62:7149-7153).

Application

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Biochem/physiol Actions

Protein Target: Abl

Target Sub-Family: TK

Packaging

Also available in 250μg size as catalog # 14-750M - Call for pricing and availability.

Physical form

50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.

Ni²⁺/NTA-agarose

Preparation Note

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Analysis Note

Routinely evaluated by phosphorylation of Abltide.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable