16-452

4G10^® Platinum, Anti-Phosphotyrosine Antibody, Biotin Conjugate

clone 4G10®, Upstate^®, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: biotin conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4G10®, monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG2bκ
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PID1(55022)

Description

General description

The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. Now Millipore has made the best even better with 4G10 Platinum. We pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20, to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone is capable.

Varies

Immunogen

The immunogen for 4G10 was phospho-tyramine coupled to KLH, while the immunogen for PY20 was phosphotyrosine conjugated to a carrier protein.

Application

4G10 Platinum, Anti-Phosphotyrosine, Biotin Conjugate detects levels of Phosphotyrosine proteins & has been published & validated for use in IP.

Physical form

100 μL of a proprietary mixture of biotin conjugated, protein G purified mouse monoclonals 4G10 (IgG2bκ) and PY20 (IgG2b) in PBS, pH 7.4 with 0.05% sodium azide.

Preparation Note

Stable for 6 months at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
NOTE: DO NOT FREEZE.

Analysis Note

Western Blot Analysis:
A dilution of 1:1000-2000 of this antibody detected tyrosine-phosphorylated proteins in a modified RIPA lysate from human HEK293 cells treated with 100 nM insulin.

Legal Information

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable