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HCYP3MAG-63K

MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel III - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

Synonym(s):

Human cytokine multiplex kit, Luminex® human cytokine immunoassay, Millipore human cytokine panel

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
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Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 92-106%
standard curve range: 2.0-200,000 pg/mL

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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This Item
HCYP2MAG-62KHCYP4MAG-64KHCYTA-60K-PX38
MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel III - Immunology Multiplex Assay Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

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HCYP3MAG-63K

MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel III - Immunology Multiplex Assay

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MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel II - Immunology Multiplex Assay

MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel IV The Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 21 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples.

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MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A 38 Plex Premixed Magnetic Bead Panel - Immunology Multiplex Assay

Millipore

HCYTA-60K-PX38

MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A 38 Plex Premixed Magnetic Bead Panel - Immunology Multiplex Assay

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 92-106%, standard curve range: 2.0-200,000 pg/mL

assay range

accuracy: 81-110%, sensitivity: 0.4-55.8 pg/mL, standard curve range: 1.0-100,000 pg/mL

assay range

accuracy: 101%
(BAFF/Blys), accuracy: 101%
(BRAK/CSCL14), accuracy: 101%
(IL-28B), accuracy: 102%
(MPIF-1/CL23), accuracy: 106%
(IL-19), accuracy: 110%
(HCC-4/CCL16), accuracy: 110%
(IL-34), accuracy: 89%
(IL-32α), accuracy: 95%
(IL-35), accuracy: 99%
(IL-37), sensitivity: 0.30 ng/mL
(YKL40/CHI3L1), sensitivity: 0.8 ng/mL
(IL-24), sensitivity: 0.8 ng/mL
(IL-35), sensitivity: 0.9 ng/mL
(BAFF/BLyS), sensitivity: 10.5 ng/mL
(HMGB1/HMG1), sensitivity: 100.8 pg/mL
(CCL28), sensitivity: 121.5 pg/mL
(IL-34), sensitivity: 17.2 pg/mL
(IL-28B/IFN-λ3), sensitivity: 248.2 pg/mL, sensitivity: 27.7 pg/mL
(IL-14/α-Taxilin), sensitivity: 28.4 pg/mL
(HCC-4), sensitivity: 3.5 pg/mL
(IL-36β), sensitivity: 32.1 pg/mL
(IL-32α), sensitivity: 33.2 pg/mL
(IL-38), sensitivity: 375.6 pg/mL
(IFNβ), sensitivity: 4.7 pg/mL
(CXCL16), sensitivity: 5.2 pg/mL
(IL-37/IL-1F7), sensitivity: 6.6 pg/mL
(MIP-4/PARC), sensitivity: 607 pg/mL
(BRAK), sensitivity: 7.8 pg/mL
(MPIF-1), standard curve range: 0.1-100 ng/mL
(YKL40/CHI3L1), standard curve range: 0.44-400 ng/mL
(BAFF/Blys), standard curve range: 0.7-750 ng/mL
(IL-24), standard curve range: 0.8-800 ng/mL
(IL-35), standard curve range: 10-10,000 pg/mL
(MPIF-1/CL23), standard curve range: 2-2,000 pg/mL
(IL-36β), standard curve range: 2.0-2,000 ng/mL
(HMGB1), standard curve range: 24-25,000 pg/mL
(IL-14), standard curve range: 244-250,000 pg/mL
(IL-19), standard curve range: 29-30,000 pg/mL
(IL-28B), standard curve range: 3-3,000 pg/mL
(CXCL16), standard curve range: 39-40,000 pg/mL
(IL-32α), standard curve range: 488-500,000 pg/mL
(BRAK/CXCL14), standard curve range: 488-500,000 pg/mL
(IFNβ), standard curve range: 49-50,000 pg/mL
(IL-38), standard curve range: 5-5,000 pg/mL
(IL-37), standard curve range: 5-5,000 pg/mL
(MIP-4/PARC/CCL18), standard curve range: 98-100,000 pg/mL
(CCL28), standard curve range: 98-100,000 pg/mL
(IL-34)

assay range

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

-

General description

“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.

The MILLIPLEX® Human Cytokine / Chemokine Panel III enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression.

The
Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Application

  • Analytes: GCP2, HCC-1, I-TAC, IL-11, IL-29, Lymphotactin, M-CSF, MIG, MIP-3α, MIP-3β, NAP2
  • Recommended Sample type: Serum, plasma, and cell culture supernatants
  • Recommended Sample dilution: Neat plasma or serum. A 1:100 dilution is required for NAP-2 and HCC-1 for serum and plasma samples
  • Assay Run Time: Overnight or two-hour primary incubation. For best results, an overnight incubation is recommended
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Obesity, Metabolic Disorders, Inflammation & Autoimmune Mechanisms

Biochem/physiol Actions

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please note: Sample Type must be selected when configuring this kit. HCC-1/CCL14a and NAP-2/CXCL7 cannot be plexed with other analytes for serum/plasma.
Sensitivity: Refer to kit protocol for sensitivities of individual cytokines/chemokines.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kristen M Reeder et al.
Mucosal immunology, 11(5), 1352-1362 (2018-06-17)
Asthmatics sensitized to fungi are reported to have more severe asthma, yet the immunopathogenic pathways contributing to this severity have not been identified. In a pilot assessment of human asthmatics, those subjects sensitized to fungi demonstrated elevated levels of the
Marie L Landry et al.
The Journal of infectious diseases, 217(6), 897-905 (2017-12-28)
Despite the high burden of respiratory infection and the importance of early and accurate diagnosis, there is no simple diagnostic test to rule in viral infection as a cause of respiratory symptoms. We performed RNA sequencing on human nasal epithelial
Alice L den Hertog et al.
PloS one, 10(6), e0129552-e0129552 (2015-06-27)
Many patients treated for tuberculosis (TB) in low and middle income countries are treated based on clinical suspicion without bacteriological confirmation. This is often due to lack of rapid simple accurate diagnostics and low healthcare provider confidence in the predictive
Matthew S Godwin et al.
JCI insight, 4(21) (2019-09-25)
Severe asthma with fungal sensitization (SAFS) defines a subset of human asthmatics with allergy to 1 or more fungal species and difficult-to-control asthma. We have previously reported that human asthmatics sensitized to fungi have worse lung function and a higher
Matthew S Godwin et al.
American journal of physiology. Lung cellular and molecular physiology, 320(3), L393-L404 (2020-12-17)
Individuals that present with difficult-to-control asthma and sensitivity to one or more fungal species are categorized as a subset of severe asthma patients belonging to a group herein referred to as severe asthma with fungal sensitization (SAFS). We have previously
View All Related Papers

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Protocol- HCYP3MAG-63K
Quality Control Ranges: Milliplex Human Cytokine/Chemokine Panel III
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