This product has not been tested for use in cell culture. Currently, a cell culture tested caffeine product is not available. However, this item has been used successfully in cell culture applications by other researchers. Please refer to the PEER REVIEWED PAPERS section above for various publications. Please see the link below to review one example:
https://pmc.ncbi.nlm.nih.gov/articles/PMC4216694/
C0750
Caffeine
powder, ReagentPlus®
Synonym(s):
Guaranine, Methyltheobromine, 1,3,7-Trimethylxanthine
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5 G
$39.30
100 G
$70.80
500 G
$248.00
1 KG
$431.00
About This Item
Empirical Formula (Hill Notation):
C8H10N4O2
CAS Number:
Molecular Weight:
194.19
UNSPSC Code:
12352210
NACRES:
NA.21
PubChem Substance ID:
EC Number:
200-362-1
Beilstein/REAXYS Number:
17705
MDL number:
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Product Name
Caffeine, powder, ReagentPlus®
InChI key
RYYVLZVUVIJVGH-UHFFFAOYSA-N
InChI
1S/C8H10N4O2/c1-10-4-9-6-5(10)7(13)12(3)8(14)11(6)2/h4H,1-3H3
SMILES string
CN1C(=O)N(C)c2ncn(C)c2C1=O
product line
ReagentPlus®
form
powder
mp
234-236.5 °C (lit.)
solubility
H2O: 15 mg/mL
Quality Level
Gene Information
human ... ADORA1(134), ADORA2A(135), ADORA2B(136), ADORA3(140), PDE4B(5142), PRKAR1A(5573), PRKAR1AP(5574), PRKAR1B(5575), PRKAR2A(5576), PRKAR2B(5577)
mouse ... Adora2b(11541)
rat ... Adora1(29290), Adora2a(25369)
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This Item | C7731 | C-051 | C6035 |
|---|---|---|---|
| mp 234-236.5 °C (lit.) | mp 234-236.5 °C (lit.) | mp - | mp - |
| product line ReagentPlus® | product line - | product line - | product line - |
| form powder | form crystalline, powder | form liquid | form - |
| solubility H2O: 15 mg/mL | solubility H2O: soluble 18.7 g/L at 16 °C | solubility - | solubility - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| Gene Information human ... ADORA1(134), ADORA2A(135), ADORA2B(136), ADORA3(140), PDE4B(5142), PRKAR1A(5573), PRKAR1AP(5574), PRKAR1B(5575), PRKAR2A(5576), PRKAR2B(5577) | Gene Information human ... ADORA1(134), ADORA2A(135), ADORA2B(136), ADORA3(140), PDE4B(5142) | Gene Information human ... ADORA1(134), ADORA2A(135), ADORA2B(136), ADORA3(140) | Gene Information human ... ADORA1(134), ADORA2A(135), ADORA2B(136), ADORA3(140) |
Application
Biochem/physiol Actions
Caffeine is a natural stimulant that is most commonly found in coffee, tea and various other drinks. It is known for its effect on the central nervous system. It is also associated with insomnia, increased heart rate, digestive issues, creating anxiety and jitteriness. Caffeine is also associated with withdrawal symptoms like headaches, fatigue, irritability.
Disclaimer
Keep tightly sealed.
Features and Benefits
High purity product that can be used readily for HPLC applications along with other research applications.
General description
Caffeine is a widely ingested pharmacologically active substance and is known to affect the central nervous system. It belongs to the class of methylxanthines.[4] It is a naturally occurring alkaloid found in coffee, cocoa and other plants. Caffeine might be associated with osteoporosis, cardiovascular risk, arrhythmia and gastrointestinal disturbances.[5] Caffeine is used to treat orthostatic hypotension,[6] bronchopulmonary dysplasia and apnea of prematurity (AOP).[7]
Legal Information
ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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A simple method for immunohistochemical staining of zebrafish brain sections for c-fos protein expression
Chatterjee D, et al.
Zebrafish, 12(6), 414-420 (2015)
Effects of caffeine and chlorogenic acid on propidium iodide accessibility to DNA: consequences on genome size evaluation in coffee tree
Noirot M, et al.
Annals of Botany, 92(2), 259-264 (2003)
Orthostatic hypotension in the elderly: diagnosis and treatment
Gupta V and Lipsitz LA
The American Journal of Medicine, 120(10), 841-847 (2007)
Effects of caffeine on human health
Nawrot P, et al.
Food Additives and Contaminants, 20(1), 1-30 (2003)
L Keskintepe et al.
Biology of reproduction, 55(2), 333-339 (1996-08-01)
The objective was to establish an in vitro system in which bovine oocytes can be matured, fertilized, and cultured up to the blastocyst stage without support of serum, BSA, or somatic cells. Media consisted of modified tissue culture medium 199
Protocols
Strat-M® synthetic membrane mimics the human skin for In-vitro permeation testing (IVPT) of topical and transdermal formulations
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Is this cell culture grade caffeine?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How do I make a stock solution of caffeine catalog number C0750?
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The stock solutions of caffeine are prepared by dissolving the appropriate amounts of caffeine in distilled water, ethanol, methanol, or chloroform. This product is tested for solubility at 15 mg/mL in water. Please note that the choice of solvent and concentration would depend on the specific application requirements.
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