E8008

Ethylenediaminetetraacetic acid solution

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

• Synonym(s): EDTA solution
• Empirical Formula (Hill Notation): C₁₀H₁₆N₂O₈
• CAS Number: 60-00-4
• Molecular Weight: 292.24
• Beilstein: 1716295
• MDL number: MFCD00003541
• UNSPSC Code: 12352204
• eCl@ss: 39030907
• PubChem Substance ID: 24894664
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• product line: BioReagent
• form: solution
• concentration: 0.02% in DPBS (0.5 mM)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• pH: 7.1-7.7
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
• InChI key: KCXVZYZYPLLWCC-UHFFFAOYSA-N

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations.[1] Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia.[2] EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques.[3] Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.[4] It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.[5]

Application

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture.[6] It has also been used to detach embryonic stem cells (ESCs) from matrigel[7] and primary human fibroblasts.[8]

Physical form

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable