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E8008

Sigma-Aldrich

Ethylenediaminetetraacetic acid solution

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

EDTA solution

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100 ML
$28.20

About This Item

Empirical Formula (Hill Notation):
C10H16N2O8
CAS Number:
Molecular Weight:
292.24
Beilstein:
1716295
MDL number:
UNSPSC Code:
12352204
eCl@ss:
39030907
PubChem Substance ID:
NACRES:
NA.75

$28.20


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sterility

sterile-filtered

product line

BioReagent

form

solution

concentration

0.02% in DPBS (0.5 mM)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.1-7.7

shipped in

wet ice

storage temp.

2-8°C

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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This Item
EDS43178803620
technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

electrophoresis: suitable

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

product line

BioReagent

product line

BioUltra

product line

-

product line

-

form

solution

form

powder

form

powder

form

powder

pH

7.1-7.7

pH

2.5 (23 °C, 10 g/L)

pH

-

pH

-

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations.[1] Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia.[2] EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques.[3] Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.[4] It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.[5]

Application

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture.[6] It has also been used to detach embryonic stem cells (ESCs) from matrigel[7] and primary human fibroblasts.[8]

Physical form

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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