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MilliporeSigma

HT901

Crystal Violet Solution

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8 FL OZ

$101.00

$101.00


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About This Item

Empirical Formula (Hill Notation):
C25H30ClN3
CAS Number:
Molecular Weight:
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3580948

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InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

form

solution

shelf life

Expiry date on the label

IVD

for in vitro diagnostic use

concentration

2.3%

technique(s)

microbe id | staining: suitable

pH

4.6

application(s)

hematology
histology

storage temp.

room temp

Quality Level

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1 of 4

This Item
HT90132V5265C0775
technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

concentration

2.3%

concentration

2.3%

concentration

1%

concentration

-

form

solution

form

solution

form

aqueous solution

form

powder

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

-

IVD

-

shelf life

Expiry date on the label

shelf life

Expiry date on the label

shelf life

-

shelf life

-

Application

Kit component of Gram Stain kits HT90A and HT90T (for staining of heat-fixed films and tissue, respectively).

Other Notes

HT901 is composed of 2.3% certified crystal violet, 0.1% ammonium oxalate monohydrate, and 20% ethyl alcohol, SD3A.

signalword

Warning

Hazard Classifications

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 2

flash_point_f

100.0 °F - closed cup

flash_point_c

37.8 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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W R Richter et al.
Journal of applied microbiology, 128(4), 1050-1059 (2019-11-30)
The purpose of this study was to evaluate the effects of altered environmental conditions on the persistence of Francisella tularensis bacteria and Venezuelan equine encephalitis virus (VEEV), on two material types. Francisella tularensis (F.t.) and VEEV were inoculated (c. 1 × 108 colony-forming
Chunmin Ge et al.
Hepatology (Baltimore, Md.), 69(6), 2608-2622 (2019-01-30)
Replication fork stability during DNA replication is vital for maintenance of genomic stability and suppression of cancer development in mammals. ATR (ataxia-telangiectasia mutated [ATM] and RAD3-related) is a master regulatory kinase that activates the replication stress response to overcome replication
Hansjörg Hufnagel et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 17(8), 1411-1417 (2009-06-18)
The understanding of internalization pathways of lipo- or polyplexes is crucial for engineering successful reagents for nonviral gene transfection. A known inhibitor of fluid phase endocytosis (FPE), rottlerin, was used to quantify the contribution of this pathway by flow cytometric
Kai-Feng Hung et al.
International journal of biological sciences, 15(5), 1080-1090 (2019-06-12)
Up-regulation of ASB6 has been previously associated with late-stage and poor prognosis of oral squamous cell carcinoma (OSCC) patients. To explore the cellular and molecular basis of how ASB6 enhances the malignancy of OSCC, we employed the clonogenicity and migration
Danuta Martuszewska et al.
PloS one, 4(2), e4350-e4350 (2009-02-06)
The Tensin family of intracellular proteins (Tensin1, -2, -3 and -4) are thought to act as links between the extracellular matrix and the cytoskeleton, and thereby mediate signaling for cell shape and motility. Dysregulation of Tensin expression has previously been

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Questions

1–4 of 4 Questions  
  1. Is Product HT901, Crystal violet solution, suitable for colony staining, migration or plaque staining?

    1 answer
    1. We recommend Product No. C0775 (Crystal Violet, certified by the Biological Stain Commission), or Product No. 93770 (Tuerk's solution) for these stains, rather than diluting down the relatively high concentration of Crystal Violet (2.3%) in HT901 to approximately 0.1%.  Product HT901 was formulated for use in the Gram Stain procedure. The product's performance has not been tested for use in colony staining, migration or plaque assays.

      Helpful?

  2. What is the formulation of Product HT901, Crystal violet solution?

    1 answer
    1. HT901 is composed of:certified Crystal Violet, 2.3%Ammonium Oxalate, 0.1%20% denatured ethyl alcohol.

      Helpful?

  3. Is Product HT901, Crystal violet solution, used as part of a kit?

    1 answer
    1. Although available separately, this solution is also a component of Gram Stain kits HT90A and HT90T (for staining of heat-fixed films and tissue, respectively).

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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