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R8508

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

buffered aqueous solution

Synonym(s):

Transfer RNA, tRNA

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About This Item

CAS Number:
9014-25-9
UNSPSC Code:
12352106
NACRES:
NA.55
MDL number:
MFCD00132200

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Product Name

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae), buffered aqueous solution

grade

Molecular Biology

form

buffered aqueous solution

concentration

9-11 mg/mL

foreign activity

DNase, Nickase, none detected

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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This Item
R5636R8759R4752
Ribonucleic acid, transfer from baker’s yeast (S. cerevisiae) buffered aqueous solution

Sigma-Aldrich

R8508

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

Ribonucleic acid, transfer from baker’s yeast (S. cerevisiae) buffered aqueous solution

Sigma-Aldrich

R5636

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

Ribonucleic acid, transfer from baker’s yeast (S. cerevisiae) Type X-SA, lyophilized powder

Sigma-Aldrich

R8759

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

Sigma-Aldrich

Sigma-Aldrich

R4752

Ribonucleic acid, transfer from bovine liver

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized powder

form

lyophilized powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

-

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

concentration

9-11 mg/mL

concentration

≥9 mg/mL

concentration

-

concentration

-

Application

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription.[1] It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.[2]
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation.

Biochem/physiol Actions

Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.[3]

General description

Transfer RNA (tRNA) is isolated from baker′s yeast.
Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.[3]

Preparation Note

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000420954
0000420962
0000464636
0000407651
0000420954

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Patrick Lonergan et al.
Molecular reproduction and development, 66(3), 297-305 (2003-09-23)
In the cyclic cow, final maturation of the ovulatory follicle is initiated by the preovulatory luteinizing hormone (LH) surge. During the subsequent 24 hr period, the oocyte nucleus undergoes meiotic progression to metaphase II and several changes in cytoplasmic organization
Encyclopedia of Agriculture and Food Systems (2015)
Daniel Castro-Roa et al.
Nature chemical biology, 9(12), 811-817 (2013-10-22)
Fic proteins are ubiquitous in all of the domains of life and have critical roles in multiple cellular processes through AMPylation of (transfer of AMP to) target proteins. Doc from the doc-phd toxin-antitoxin module is a member of the Fic
Takamasa Hirano et al.
RNA (New York, N.Y.), 20(8), 1223-1237 (2014-06-11)
Small RNAs mediate gene silencing by binding Argonaute/Piwi proteins to regulate target RNAs. Here, we describe small RNA profiling of the adult testes of Callithrix jacchus, the common marmoset. The most abundant class of small RNAs in the adult testis
Karen Yap et al.
Molecular cell, 72(3), 525-540 (2018-10-16)
Functions of many long noncoding RNAs (lncRNAs) depend on their ability to interact with multiple copies of specific RNA-binding proteins (RBPs). Here, we devised a workflow combining bioinformatics and experimental validation steps to systematically identify RNAs capable of multivalent RBP
View All Related Papers

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