The integrity of Spermidine can be verified by Gas chromatography. The following publication may be of interest for protocol reference on Gas chromatography analysis of Spermidine.
1. J Mass Spectrom. 2009 Aug;44(8):1203-10. doi: 10.1002/jms.1597.
2. A quantitative GC-MS method for three major polyamines in postmortem brain cortex, PMID: 19514045
S0266
Spermidine
BioReagent, Molecular Biology, suitable for cell culture, ≥98%
Synonym(s):
1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane
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1 G
$88.00
5 G
$254.00
25 G
$1,020.00
$88.00
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About This Item
Linear Formula:
NH2(CH2)3NH(CH2)4NH2
CAS Number:
Molecular Weight:
145.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
EC Number:
204-689-0
Beilstein/REAXYS Number:
1698591
MDL number:
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Product Name
Spermidine, BioReagent, Molecular Biology, suitable for cell culture, ≥98%
InChI key
ATHGHQPFGPMSJY-UHFFFAOYSA-N
InChI
1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
SMILES string
NCCCCNCCCN
grade
Molecular Biology
product line
BioReagent
assay
≥98%
form
solid or liquid
mol wt
145.25 (AMU)
technique(s)
cell culture | mammalian: suitable
refractive index
n20/D 1.479 (lit.)
density
0.925 g/mL at 25 °C (lit.)
foreign activity
DNase, RNase, and protease, none detected
storage temp.
room temp
Quality Level
Gene Information
rat ... Ppm1a(24666)
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This Item | 85558 | S2626 | 49761 |
|---|---|---|---|
| assay ≥98% | assay ≥99.5% (GC) | assay ≥99% (GC) | assay ≥97.5% (NT), ≥99.0% (GC) |
| form solid or liquid | form - | form solid or liquid (clear) | form - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| technique(s) cell culture | mammalian: suitable | technique(s) ChIP: suitable, buffer exchange: suitable | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. 2-8°C |
| mol wt 145.25 (AMU) | mol wt - | mol wt - | mol wt - |
Application
Biochem/physiol Actions
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T4 polynucleotide kinase activity.
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.
Disclaimer
Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.
General description
Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.[5]
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signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
233.6 °F - closed cup
flash_point_c
112 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Can the reconstituted S0266 solution, which has been sitting at room temperature for 3 months, still be used in CUT&Tag assays to stabilize nuclei? Also, how can the integrity of spermidine be tested? If there's no more spermidine available to test the solution, should more be purchased?
1 answer-
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What is the solvent that spermidine is dissolved in?
1 answer-
This product is a neat or pure liquid and has not been diluted in a solvent. However, to further dilute this product, it is recommended to use water at 50 mg/mL water.
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How to store S0266-5G? which comes as liquid and what is the molaritty of it? which solvent to use to dilute it?
1 answer-
This product can be stored in room temperature in its original format. The concentration of this product can be determined using the lot specific purity reported on the Certificate of Analysis, the density, and the compound molecular weight. This product has a density of 0.925 g/mL and a molecular weight of 145.25 g/mol. The minimum purity is 98%, so the molarity will vary slightly from lot to lot. The molarity can range from 6.2M (98%) to 6.4M (100%).
Molarity=Density x 1000 x Purity/MWThis product is supplied as a liquid or solid. A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter.
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How to store S0266-1G which comes as liquid? alliquoted and store at -20C? How long it can be stored at this condition?
1 answer-
Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.
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How frequently should new solutions be made?
1 answer-
A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter. Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.
Helpful?
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Is this a spermidine solution or a neat liquid, and what is the concentration?
1 answer-
Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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