SHC005V

MISSION^® pLKO.1-puro eGFP shRNA Control Transduction Particles

Name: MISSION<SUP>®</SUP> pLKO.1-puro eGFP shRNA Control Transduction Particles
Brand: SIGMA
Price: 573 CAD

shRNA sequence targeting eGFP

Synonym(s):

MISSION^®, MISSION^® Control Transduction Particles

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$573.00

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About This Item

NACRES:

NA.51

UNSPSC Code:

41106609

Key Documents

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Product Name

MISSION^® pLKO.1-puro eGFP shRNA Control Transduction Particles, shRNA sequence targeting eGFP

product line

MISSION^®

concentration

≥1x10⁶ VP/ml (via p24 assay)

technique(s)

capture ELISA: 10⁶ TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

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Show Differences

1 of 4

This Item	SHC004V	SHC007V	SHC202V
Sigma-Aldrich SHC005V MISSION^® pLKO.1-puro eGFP shRNA Control Transduction Particles	Sigma-Aldrich SHC004V MISSION^® pLKO.1-puro TurboGFP^™ shRNA Control Transduction Particles	Sigma-Aldrich SHC007V MISSION^® pLKO.1-puro Luciferase shRNA Control Transduction Particles	Sigma-Aldrich SHC202V MISSION^® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Transduction Particles
product line MISSION^®	product line MISSION^®	product line MISSION^®	product line MISSION^®
concentration ≥1x10⁶ VP/ml (via p24 assay)	concentration ≥1x10⁶ VP/ml (via p24 assay)	concentration ≥1x10⁶ VP/ml (via p24 assay)	concentration ≥1x10⁶ VP/ml (via p24 assay)
technique(s) capture ELISA: 10⁶ TU/mL using p24	technique(s) capture ELISA: 10⁶ TU/mL using p24	technique(s) capture ELISA: 10⁶ TU/mL using p24	technique(s) -
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
storage temp. −70°C	storage temp. −70°C	storage temp. −70°C	storage temp. −70°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

General description

The MISSION eGFP shRNA Control Transduction Particles contain an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761). The eGFP shRNA Control Particles are useful as a positive knockdown control in experiments using cell lines expressing eGFP.
The eGFP shRNA Control Transduction Particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-eGFP shRNA (Prod. No. SHC005). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.[1] In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 10⁶ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION^® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pricing

SHM01

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SHM02

MISSION^® ExpressMag^® 96-Well Magnetic Kit, Increases transduction efficiency

SHC016V

MISSION^® pLKO.1-puro Non-Target shRNA Control Transduction Particles, Targets no known genes from any species

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

02271712MN

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The SCN9A channel and plasma membrane depolarization promote cellular senescence through Rb pathway.

Marine Warnier et al.

Aging cell, 17(3), e12736-e12736 (2018-02-16)

Oncogenic signals lead to premature senescence in normal human cells causing a proliferation arrest and the elimination of these defective cells by immune cells. Oncogene-induced senescence (OIS) prevents aberrant cell division and tumor initiation. In order to identify new regulators

Cell-cycle dependent expression of a translocation-mediated fusion oncogene mediates checkpoint adaptation in rhabdomyosarcoma.

Kikuchi K, Hettmer S, Aslam MI, et al.

PLoS Genetics, 10(1), e1004107-e1004107 (2014)

Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery.

R Zufferey et al.

Journal of virology, 72(12), 9873-9880 (1998-11-13)

In vivo transduction of nondividing cells by human immunodeficiency virus type 1 (HIV-1)-based vectors results in transgene expression that is stable over several months. However, the use of HIV-1 vectors raises concerns about their safety. Here we describe a self-inactivating

A precision oncology approach to the pharmacological targeting of mechanistic dependencies in neuroendocrine tumors.

Mariano J Alvarez et al.

Nature genetics, 50(7), 979-989 (2018-06-20)

We introduce and validate a new precision oncology framework for the systematic prioritization of drugs targeting mechanistic tumor dependencies in individual patients. Compounds are prioritized on the basis of their ability to invert the concerted activity of master regulator proteins

Roles of polypyrimidine tract binding proteins in major immediate-early gene expression and viral replication of human cytomegalovirus.

Ruth S Cruz Cosme et al.

Journal of virology, 83(7), 2839-2850 (2009-01-16)

Human cytomegalovirus (HCMV), a member of the beta subgroup of the family Herpesviridae, causes serious health problems worldwide. HCMV gene expression in host cells is a well-defined sequential process: immediate-early (IE) gene expression, early-gene expression, DNA replication, and late-gene expression.

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MISSION® pLKO.1-puro eGFP shRNA Control Transduction Particles