LC/MS Analysis of Methanesulfonic Acid in Busulfan Injection on SeQuant^® ZIC-HILIC

column

SeQuant ZIC-HILIC, PEEK, 150 x 4.6 mm, 3.5 μm, 200 Å (1.50444.0001)

mobile phase

[A] 100 mM ammonium acetate, pH 4.5; [B] acetonitrile; (20:80, A:B)

flow rate

0.5 mL/min

pressure

826 psi (57 bar)

column temp.

40 °C

detector

Shimadzu Prominence, RI (cell temperature  40 °C, Range 1.0, 10 μL cell)

injection

20 μL

sample

0.2 mg/ml in mobile phase