This product has not been tested for labeling bacteria internally, including Staphylococcus aureus. Therefore, no data is available for this application. A literature search is recommended for specific applications.
46950
Fluorescein 5(6)-isothiocyanate
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Fluorescein 5(6)-isothiocyanate
Synonyme(s) :
FITC, Fluorescein isothiocyanate
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
50 MG
52,70 $
250 MG
207,00 $
1 G
610,00 $
A propos de cet article
Formule empirique (notation de Hill) :
C21H11NO5S
Numéro CAS:
Poids moléculaire :
389.38
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
248-207-7
Beilstein/REAXYS Number:
1407295
MDL number:
Passer à
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderNom du produit
Fluorescein 5(6)-isothiocyanate, BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
product line
BioReagent
assay
≥90% (HPLC)
Quality Level
form
powder
mol wt
389.38 g/mol
color
orange to dark orange
yellow to dark yellow
solubility
acetone: 0.1 g/10 mL, clear to very slightly hazy
fluorescence
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
suitability
suitable for fluorescence
storage temp.
−20°C
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Catégories apparentées
1 of 4
Cet article | F3651 | F7250 | F4274 |
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| fluorescence λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0 | fluorescence - | fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 492 nm; λem 518 nm (green) |
| assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥97.5% (HPLC) |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| product line BioReagent | product line - | product line - | product line - |
Application
Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography
General description
Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Wenhuo Hu et al.
Blood, 125(14), 2206-2216 (2015-01-31)
Hematopoietic stem cells (HSCs) possess the ability to generate all hematopoietic cell types and to self-renew over long periods, but the mechanisms that regulate their unique properties are incompletely understood. Herein, we show that homozygous deletion of the miR-29a/b-1 bicistron
Marina Vivarelli et al.
Kidney international, 87(3), 602-609 (2015-01-08)
Alloimmune antenatal membranous nephropathy (MN) during pregnancy results from antibodies produced by a neutral endopeptidase (NEP)-deficient mother. Here we report two recent cases that provide clues to the severity of renal disease. Mothers of the two children had circulating antibodies
Benoit Malleret et al.
Blood, 125(8), 1314-1324 (2014-11-22)
Plasmodium vivax merozoites only invade reticulocytes, a minor though heterogeneous population of red blood cell precursors that can be graded by levels of transferrin receptor (CD71) expression. The development of a protocol that allows sorting reticulocytes into defined developmental stages
Eddie A James et al.
Arthritis & rheumatology (Hoboken, N.J.), 66(7), 1712-1722 (2014-03-26)
Rheumatoid arthritis (RA) is thought to be a T cell-mediated disease, based on its strong association with HLA class II alleles, clinical responsiveness to T cell-directed therapies, and the presence of CD4+ T cells in rheumatoid joints. The presence of
Retinoic acid primes human dendritic cells to induce gut-homing, IL-10-producing regulatory T cells.
G Bakdash et al.
Mucosal immunology, 8(2), 265-278 (2014-07-17)
The vitamin A metabolite all-trans retinoic acid (RA) is an important determinant of intestinal immunity. RA primes dendritic cells (DCs) to express CD103 and produce RA themselves, which induces the gut-homing receptors α4β7 and CCR9 on T cells and amplifies
-
excuse me， can the product of Fluorescein 5(6)-isothiocyanate be used to lable bacteria，such as Staphylococcus aureus？ thanks
1 answer-
Helpful?
-
-
Is it suitable for fluorescence microscopy?
1 answer-
This product is tested for fluorescent labeling of proteins. While not tested specifically for use in fluorescent microscopy, this compound is routinely used in cell-based studies applying this technique.
Please see the link below to review the Peer Reviewed Publications:
https://www.sigmaaldrich.com/search/46950?focus=papers&page=1&perpage=30&sort=relevance&term=46950&type=citation_searchHelpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique