FITC is not membrane permeable in healthy cells, it binds to surface proteins. Conjugates such as FITC-Dextran is an example of a more cell-permeable option.
F7250
Fluorescein isothiocyanate isomer I
≥90% purity (HPLC), powder
Synonyme(s) :
FITC, Fluorescein 5-isothiocyanate
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A propos de cet article
Formule empirique (notation de Hill) :
C21H11NO5S
Numéro CAS:
Poids moléculaire :
389.38
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
222-042-0
MDL number:
Beilstein/REAXYS Number:
1407295
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Laissez-nous vous aiderNom du produit
Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
assay
≥90% (HPLC)
form
powder
technique(s)
titration: suitable
color
orange to dark orange
mp
>360 °C (lit.)
solubility
acetone: 1 mg/mL
fluorescence
λex 492 nm; λem 518 nm (green)
suitability
suitable for protein labeling
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
Quality Level
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Cet article | F4274 | 34321-M | 46951 |
|---|---|---|---|
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) - | technique(s) - |
| suitability suitable for protein labeling | suitability - | suitability - | suitability - |
| fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 492 nm; λem 518 nm (green) | fluorescence - | fluorescence λex 492 nm; λem 518 nm (green), λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0 |
| color orange to dark orange | color yellow to very dark yellow | color orange-red | color - |
| solubility acetone: 1 mg/mL | solubility acetone: soluble 10 mg/mL | solubility DMSO: 5 mg/mL, DMF: soluble, aqueous buffer, pH ≥ 9.0: soluble | solubility acetone: soluble 0.1 g/10 mL |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
Application
Réactif pour le marquage FITC des protéines; le microséqueçage de protéines et de peptides (HPLC)
Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins[1][2][3], a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
Biological applications include use as a fluorescent labeling reagent for proteins[1][2][3], a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
General description
Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[4][5]
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.[6]
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[4][5]
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.[6]
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
A Ramseier et al.
Electrophoresis, 19(16-17), 2967-2975 (1998-12-31)
Amines can easily be derivatized with fluorescein isothiocyanate isomer I (FITC) and analyzed by capillary electrophoresis (CE) using alkaline buffers with or without dodecyl sulfate micelles. This paper reports the CE analysis of FITC-derivatized amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and beta-phenylethylamine in
Chromatographically pure fluorescein and tetramethylrhodamine isothiocyanates.
L C FELTON et al.
Analytical biochemistry, 2, 178-180 (1961-04-01)
S E Macatonia et al.
Immunology, 59(4), 509-514 (1986-12-01)
Lymph node cells taken 24 hr after skin-painting mice with the contact sensitizer fluorescein isothiocyanate (FITC) induce delayed-type hypersensitivity in recipient mice. Skin-painting increased the number of dendritic cells (DC) in the draining lymph nodes without significantly changing the number
Test of a two-pathway model for small-solute exchange across the capillary wall.
Fu BM
The American Journal of Physiology, 274, H2062-H2062 (1998)
Human monoclonal autoantibodies specific for the bullous pemphigoid antigen 1 (BPAg 1).
Peyron E
Journal of Immunology, 153, 1333-1333 (1994)
Articles
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Contenu apparenté
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Are cell membranes of living cells permeable for FITC?
1 answer-
Helpful?
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Is the intensity of FITC (e.g., Product No. F7250) pH dependent?
1 answer-
Yes; many articles have reported the pH dependence of the fluorescence of fluorescein derivatives. See the following references: Archives Biochem. Biophys., 133, 263 (1969); J. Immunol., 95, 1165 (1966); Archives Biochem. Biophys., 73, 1 (1958); Standard test for labeling efficiency of FITC with BSA: NCCLS Approved Standard: ASM-1, 2nd ed., June, 1975; Characterization of FITC-dyes by IR and TLC: Analytical Biochem., 29, 526 (1969); Fluorescent labeling of proteins: Proc. Nat. Acad. Sci., 72, 459 (1975); Amer. J. Pathol., 34, 1081 (1958); Fluorescent labeling of a lectin: Meth. Enzymol., 81, 65 (1982).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product No. F7250, Fluorescein isothiocyanate isomer I, pharmaceutical grade?
1 answer-
No. The product is considered suitable for research use only.
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How stable is FITC Isomer 1 (e.g., Product No. F7250) once conjugated to an antibody?
1 answer-
FITC can photo bleach fairly rapidly. The prepared solution should be stored in a dark container and protected from light. When viewing the antibody staining under a microscope, it is advisable to use subdued fluorescent light when possible. If the fluorescent light stays at one position for too long, photo bleaching is noticeable.
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After the FITC (e.g., Product No. F7250) is conjugated to the protein, what is the solution stability of the conjugated protein?
1 answer-
The stability of the conjugated protein will be essentially the stability of the unconjugated protein. Some conjugated proteins might be stable when stored at -20°C and other conjugated proteins will have better solution stability at 2-8°C If the protein concentration is too low, this also has a negative effect upon the stability of the conjugated protein.
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