AB5970

Anti-Dishevelled-1 Antibody

Chemicon^®, from rabbit

• Synonyme(s) : DSH, DVL1, DSH Homolog 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: affinity purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Fabricant/nom de marque: Chemicon^®
• Technique(s): western blot: suitable
• Entrée: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• Numéro d'accès NCBI: NM_004421.2; NM_181870.1; NM_182779.3
• Numéro d'accès UniProt: O14640
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DVL1(1855)

Description

Description générale

85 kDa

DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation. The protein acts as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 mutation is likely responsible for transformation in neuroblastomas, and the Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1 gene, thus DVL1 maybe important in these diseases as well.

Immunogène

Synthetic peptide corresponding to amino acids 557-571 of human dishevelled-1 protein.

Application

Anti-Dishevelled-1 Antibody is an antibody against Dishevelled-1 for use in WB.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells

Western blot: 1:500 using ECL. The antibody reacts with a protein of ~85kDa. Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

Actions biochimiques/physiologiques

Dishevelled-1 protein (dvl-1). By Western blot the antibody shows no cross reactivity with recombinant dishevelled -2 or dishevelled -3 protein Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against dishevelled -1 protein.

Forme physique

Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

Notes préparatoires

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

Remarque sur l'analyse

Control
SK-N-SH (neuroblastoma) whole cell lysates

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable