MAB1952Z

Anti-Integrin α3 Antibody, clone P1B5, azide free

clone P1B5, Chemicon^®, from mouse

• Synonyme(s) : CD49c, MAB1952
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: P1B5, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Chemicon^®
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin)
• Isotype: IgG1
• Numéro d'accès NCBI: NM_002204.1; NM_005501.1
• Numéro d'accès UniProt: P26006
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ITGA3(3675)

Description

Description générale

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.

Application

Anti-Integrin α3 Antibody, clone P1B5, azide free detects level of Integrin α3 & has been published & validated for use in IC, IH(P).

Research Category
Cell Structure

Research Sub Category
Integrins

Suitable for use in attachment inhibition assays using fibroblasts, most epithelial cells, activated lymphocytes on laminin, collagen and fibronectin.

Immunohistochemistry on human tonsil

fresh frozen tissue

paraffin embedded tissue; protease digestion required:

Optimal working dilutions must be determined by end user.

Actions biochimiques/physiologiques

Reacts with Human alpha3 integrin.

Forme physique

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.

Protein A purified

Notes préparatoires

Maintain at 2–8°C in undiluted aliquots for up to 6 months after date of receipt.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Remarque sur l'analyse

Control
Human foetal foreskin fibroblasts (HFFF), neuroblastomas

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable