MAB3864

Anti-DNA/Histone H1 Antibody

Chemicon^®, from mouse

• Synonyme(s) : Anti-H1.4, Anti-H1E, Anti-H1F4, Anti-H1s-4, Anti-HIST1H1E, Anti-RMNS, Anti-dJ221C16.5
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: monoclonal
• Réactivité de l'espèce (prédite par homologie): all
• Fabricant/nom de marque: Chemicon^®
• Technique(s): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable
• Isotype: IgG2a
• Numéro d'accès NCBI: NM_005321.2
• Numéro d'accès UniProt: P10412
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... H1-1(3024)

Description

Immunogène

Human Raji cell nuclei

Application

Anti-DNA/Histone H1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of DNA/Histone H1 & has been validated in ELISA, FC, ICC & IHC.

Immunocytochemistry: 5-10 μg/mL on 2% paraformaldehyde fixed cells (10 minutes at room temperature)

Immunohistochemistry: 5-10 μg/mL on paraformaldehyde or B5 fixed tissue sections.

Flow cytometry: 5-10 μg/mL using 2% paraformaldehyde fixed cells.

ELISA, direct and indirect

Optimal working dilutions must be determined by end user.

STAINING PATTERN:

Nuclear staining observed. Dense staining of nuclei. No staining of nucleoli.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Actions biochimiques/physiologiques

Reacts with DNA/Histone 1. The antibody can be used to stain nuclei of all species and to identify necrosis. By immunohistochemistry, the antibody shows dense staining of nuclei with no staining of nucleoli observed.

Forme physique

Format: Purified

Protein A purified culture supernatant. Liquid in PBS

Notes préparatoires

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable