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Merck

47608

Formaldéhyde solution

Molecular Biology, BioReagent, ≥36.0% in H2O (T)

Synonyme(s) :

Formol

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A propos de cet article

Formule linéaire :
HCHO
Numéro CAS:
50-00-0
Poids moléculaire :
30.03
UNSPSC Code:
12352114
NACRES:
NA.52
PubChem Substance ID:
329757328
MDL number:
MFCD00003274
Beilstein/REAXYS Number:
1209228
Grade:
Molecular Biology
Technique(s):
electrophoresis: suitable
Vapor pressure:
52 mmHg ( 37 °C)

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Nom du produit

Formaldéhyde solution, Molecular Biology, BioReagent, ≥36.0% in H2O (T)

InChI

1S/CH2O/c1-2/h1H2

SMILES string

[H]C([H])=O

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

grade

Molecular Biology

vapor density

1.03 (vs air)

vapor pressure

52 mmHg ( 37 °C)

product line

BioReagent

form

liquid

autoignition temp.

572 °F

contains

~10% methanol as stabilizer

concentration

≥36.0% in H2O (T)
36.0-38.0% (aldehyde method)

technique(s)

electrophoresis: suitable

impurities

≤0.03% free acid (as HCOOH)

ign. residue

≤0.05% (as SO4)

pH

2.8-4.0

density

1.09 g/mL at 20 °C
1.09 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤20 mg/kg

cation traces

Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Na: ≤100 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Zn: ≤1 mg/mL

λ

2 M in H2O

UV absorption

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

suitability

in accordance for electrophoresis of RNA in agarose gels

storage temp.

room temp

Quality Level

100

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Cet article
F877547083-U1.04003
Formaldéhyde solution Molecular Biology, BioReagent, ≥36.0% in H2O (T)

Sigma-Aldrich

47608

Formaldéhyde solution

Formaldéhyde solution Molecular Biology, 36.5-38% in H2O

Sigma-Aldrich

F8775

Formaldéhyde solution

Formaldéhyde solution stabilized with methanol, ~37 wt. % in H2O, certified reference material

Supelco

47083-U

Formaldéhyde solution

Formaldéhyde en solution (env. 37 %) GR for analysis stabilized with about 10% methanol ACS,Reag. Ph Eur

Sigma-Aldrich

1.04003

Formaldéhyde en solution (env. 37 %)

technique(s)

electrophoresis: suitable

technique(s)

ChIP: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

grade

for molecular biology

grade

for molecular biology

grade

certified reference material

grade

ACS reagent

Quality Level

100

Quality Level

100

Quality Level

300

Quality Level

400

form

liquid

form

liquid

form

-

form

liquid

density

1.09 g/mL at 20 °C, 1.09 g/mL at 25 °C (lit.)

density

1.09 g/mL at 25 °C (lit.)

density

1.09 g/mL at 25 °C (lit.)

density

1.09 g/cm3 at 20 °C, 1.09 g/mL at 25 °C (lit.)

cation traces

Ca: ≤5 mg/kg, Co: ≤1 mg/kg, Cu: ≤1 mg/kg, K: ≤20 mg/kg, Mn: ≤1 mg/kg, Ni: ≤1 mg/kg, Zn: ≤1 mg/mL, Cd: ≤1 mg/kg, Fe: ≤1 mg/kg, Na: ≤100 mg/kg, Cr: ≤1 mg/kg, Pb: ≤1 mg/kg, Mg: ≤1 mg/kg

cation traces

-

cation traces

-

cation traces

-

Application

Formaldehyde solution is suitable for:
  • denaturation and separation of RNA molecules
  • anti-bacterial treatment
  • fixation of cells
Formaldehydesolution has been used:
  • in the fixation of human spermatogonial stem cell transplantation (SSCT).[1]
  • to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.[2]
  • in fixing zebra fish larvae for neutrophil staining.[3]

Biochem/physiol Actions

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

Disclaimer

may contain precipitate (paraformaldehyde)

General description

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.[4]

Related product

Réf. du produit
Description
Tarif

signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

Classe de stockage

3 - Flammable liquids

wgk

WGK 3

flash_point_f

133.0 °F - closed cup

flash_point_c

56.11 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificats d'analyse (COA)

Lot/Batch Number
BCCN1938
BCCM9304
BCCM0733
BCCL7726
BCCL1095

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation
Onofre J, et al.
Reproductive Biomedicine Online, 40(2), 261-269 (2020)
Formaldehyde
Gerberich, H R and Seaman, G C.
Kirk-Othmer Encyclopedia of Chemical Technology (2013)
Jin Qian et al.
Virology, 474, 10-18 (2014-12-03)
Interferon-induced transmembrane (IFITM) proteins inhibit the entry of a large number of viruses. Not surprisingly, many viruses are refractory to this inhibition. In this study, we report that different strains of HIV and SIV are inhibited by human IFITM proteins
John N Griffin et al.
PLoS genetics, 11(3), e1005018-e1005018 (2015-03-11)
The production of ribosomes is ubiquitous and fundamental to life. As such, it is surprising that defects in ribosome biogenesis underlie a growing number of symptomatically distinct inherited disorders, collectively called ribosomopathies. We previously determined that the nucleolar protein, NOL11
Y Jiang et al.
Oncogene, 34(50), 6079-6091 (2015-03-10)
Epstein-Barr virus (EBV) causes human lymphoid malignancies, and the EBV product latent membrane protein 1 (LMP1) has been identified as an oncogene in epithelial carcinomas such as nasopharyngeal carcinoma (NPC). EBV can epigenetically reprogram lymphocyte-specific processes and induce cell immortalization.
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