This product contains 3.7 g/L of NaHCO3.
D5796
DMEM - high glucose
With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
Synonyme(s) :
DME, DMEM
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A propos de cet article
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
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Laissez-nous vous aiderNom du produit
Milieu d′Eagle modifié par Dulbecco (DMEM) à teneur élevée en glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Stérilité
sterile-filtered
Forme
liquid
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Composants
NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Cet article | D5671 | D6429 | D6546 |
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form liquid | form liquid |
| components NaHCO3: yes | components L-glutamine: no | components HEPES: no | components NaHCO3: yes |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
Description générale
Ce milieu DMEM à teneur élevée en glucose (« DMEM-Hi ») est un milieu complet 1x sans facteurs de supplémentation (modifications courantes) tels que L-alanyl-L-glutamine, HEPES ou pyruvate de sodium. Il diffère de la formulation DMEM-Hi d′origine en ce que le pyridoxal a été remplacé par de la pyridoxine. Le pyridoxal est un constituant instable des milieux.
Application
Le milieu de Eagle modifié par Dulbecco (DMEM) est une variante du milieu de Eagle de base (BME) qui contient quatre fois plus d'acides aminés et de vitamines. La formulation d'origine contenait 1 000 mg/l de glucose et servait à la culture de cellules embryonnaires de souris. Depuis, le milieu a été modifié à plusieurs reprises pour convenir aux primocultures de cellules de souris et de poulet ainsi que de diverses cellules normales ou transformées. Chacun de ces milieux apporte une combinaison différente de L-glutamine et de pyruvate de sodium. En outre, le taux de glucose a été augmenté à 4 500 mg/l, d'où le nom "DMEM/High" pour DMEM riche en glucose.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
used together
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Instructions
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What is the NaHCO3 concentration? Conventional cell culture requires 5% of CO2. But it will depend on the NaHCO3 concentration. Does this product use 1.2 or 3.7 g/L?
1 answer-
Helpful?
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What are the product numbers for DMEM and EMEM used to grow the 293 and 293T cell lines?
1 answer-
To grow the 293 and 293T cells, the following product recommendations are provided:
For the 293 cell line (product number 85120602), ECACC recommends using EMEM media (EBSS) with 2mM Glutamine, 1% Non Essential Amino Acids (NEAA), and 10% FCS. ECACC uses the product number M4655 for this purpose.
For the 293T cell line (product number 12022001), ECACC recommends using DMEM media with 2mM Glutamine and 10% Fetal Bovine Serum (FBS). ECACC uses the product number D5796 media for sub-culturing this cell line.
Helpful?
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What is the conc. of L glutamine
1 answer-
The concentration of L-Glutamine in this product is 0.584 g/L. Please see the link below to review the DMEM Formulation Tables: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/340/109/d5796for.pdf
Helpful?
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pH는 몇으로 조정되어 있나요?
1 answer-
The pH specification of this product is 7.0-7.6. The exact value will be reported in the lot specific Certificate of Analysis. Please see the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/coa/SIGMA/D5796/RNBL9771.
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