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Merck

D9170

Diméthylsulfoxyde

PCR Reagent

Synonyme(s) :

DMSO, Sulfoxyde de méthyle

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1 AMP

91.80 CHF

5 AMP

335.00 CHF

91.80 CHF


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A propos de cet article

Formule linéaire :
(CH3)2SO
Numéro CAS:
Poids moléculaire :
78.13
Beilstein:
506008
Numéro CE :
Numéro MDL:
Code UNSPSC :
12191502
ID de substance PubChem :
Nomenclature NACRES :
NA.25
Technique(s):
PCR: suitable
Application(s):
agriculture

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Densité de vapeur

2.7 (vs air)

Pression de vapeur

0.42 mmHg ( 20 °C)

Essai

≥99.5%

Forme

liquid

Température d'inflammation spontanée

573 °F

Durée de conservation

Recommended retest period - 2 years

Limite d'explosivité

42 %, 63 °F

Conditionnement

vial of 1 mL

Technique(s)

PCR: suitable

Impuretés

≤0.1% water
water

Couleur

colorless

Indice de réfraction

n20/D 1.479 (lit.)

pb

189 °C (lit.)

Pf

16-19 °C (lit.)

Solubilité

H2O: miscible (completely)

Densité

1.10 g/mL (lit.)

Adéquation

suitable for PCR

Application(s)

agriculture

Chaîne SMILES 

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

Clé InChI

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Cet article
3486941640-MD8418
application(s)

agriculture

application(s)

food and beverages

application(s)

-

application(s)

-

grade

PCR Reagent

grade

-

grade

ACS reagent, puriss. p.a.

grade

for molecular biology

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

200

assay

≥99.5%

assay

≥99.7%

assay

≥99.9% (GC)

assay

≥99.9%

technique(s)

PCR: suitable

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

DNA sequencing: suitable, PCR: suitable, transfection: suitable

solubility

H2O: miscible (completely)

solubility

-

solubility

-

solubility

H2O: miscible (completely)

Description générale

Dimethyl sulfoxide is a free radical scavenger and a cryopreservant. It elicits protective effect in double stranded breaks(DSBs).[1] Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination.[2] As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.[3]

Application

Dimethyl sulfoxide has been used as a component of polymerase chain reaction (PCR) mixture[4] and as PCR facilitator.[2][5]

Autres remarques

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Clause de non-responsabilité

Refroidit facilement et revient doucement à la température de la pièce. Les produits solides peuvent être reliquéfiés par chauffage à la température ambiante sans abîmer le produit.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Équipement de protection individuelle

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Certificats d'analyse (COA)

Lot/Batch Number

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and gamma-ray-irradiation, and freezing
Noda M, et al.
Scientific reports, 7(1), 8557-8557 (2017)
Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials
Ravikumar et al.
Applied Biochemistry and Biotechnology, 175(1), 3856-3862 (2015)
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
Lorenz, Todd C
Journal of Visualized Experiments, (63) (2012)
IRAP and REMAP for retrotransposon-based genotyping and fingerprinting
Kalendar R and Schulman AH
Nature Protocols, 1(5), 2478-2478 (2006)
Microtitre Plate Based Cell-SELEX Method
Shorie M and Kaur H
Bio-protocol, 8(20) (2018)

Questions

1–9 of 9 Questions  
  1. How much DMSO is in each of the vials (D9170)?

    1 answer
    1. As stated in the 'PROPERTIES' section, each vial contains 1 mL. One ampule contains 1 mL of Dimethyl Sulfoxide, and 5 amps include 5 ampules containing 1 mL of Dimethyl Sulfoxide. each.

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. Where does DMSO come from?

    1 answer
    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

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  5. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    1 answer
    1. DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

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  6. Does DMSO have any synthetic utility?

    1 answer
    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the molarity of DMSO?

    1 answer
    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  9. What is the DMSO dissolved in?

    1 answer
    1. DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

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