Accéder au contenu
Merck

F3165

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

ANTI-FLAG® M2 antibody, Mouse monoclonal

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

Slide 1 of 1
Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

0.2 MG

603.00 CHF

1 MG

1 180.00 CHF

5 MG

3 110.00 CHF

603.00 CHF

Disponible dès aujourd'huiDétails

Demander une commande groupée

A propos de cet article

NACRES:
NA.32
UNSPSC Code:
12352203

Principaux documents

Voir toute la documentation

Passer à

Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

purified by

using Protein A

species reactivity

all

concentration

3.8-4.2 mg/mL

technique(s)

western blot: 10 μg/mL (Protein A)

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Catégories apparentées

Comparer avec des articles similaires

Voir la comparaison complète

Montrer les différences

1 of 4

Cet article
F9291A9594A8592
Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Sigma-Aldrich

F3165

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

F9291

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

Sigma-Aldrich

A9594

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse

Anticorps monoclonal ANTI-FLAG® M2-peroxydase (HRP) antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

A8592

Anticorps monoclonal ANTI-FLAG® M2-peroxydase (HRP) antibody produced in mouse

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

conjugate

unconjugated

conjugate

biotin conjugate

conjugate

CY3 conjugate

conjugate

peroxidase conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

form

buffered aqueous glycerol solution

form

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

form

buffered aqueous glycerol solution

Other Notes

Lauréat du prix CiteAb 2024 récompensant les avancées des fournisseurs dans la recherche sur la maladie de Parkinson

Preparation Note

Diluer la solution d'anticorps à raison de 0,5-10 µg/ml dans une solution saline tamponnée au Tris, pH 8,0, avec 3 % de lait écrémé
Stocker l'anticorps non dilué à -20 °C en aliquotes de travail. Il n'est pas recommandé de procéder à des congélations et décongélations répétées.
Remarque : Au fil du temps, de petites quantités d'anticorps purifiés peuvent précipiter à partir de la solution en raison d'interactions hydrophobes intermoléculaires. Si un précipité est observé dans ce produit, centrifuger brièvement le flacon pour obtenir un culot contenant le précipité. Prélever le volume de solution d'anticorps souhaité dans le surnageant limpide avant utilisation. Cela ne devrait pas altérer la performance de l'anticorps purifié dans les applications de western blotting ou d'immunoprécipitation.

Application

L'anticorps monoclonal M2 ANTI-FLAG® produit chez la souris est utilisé dans les applications suivantes :
  • Immunoblotting/Immunotransfert
  • Immunoprécipitation
  • Immunocytochimie
  • Immunofluorescence
  • Dosages ELISA
  • Dosages immunoenzymatiques
  • Immunoprécipitation de la chromatine
  • Microscopie électronique
  • Cytométrie en flux
  • Tests supershift

Consultez notre portail Littérature sur le système FLAG® pour découvrir d'autres références sur cette application.

General description

L'anticorps anti-Flag M2 permet de détecter les protéines de fusion portant une étiquette Flag. Cet anticorps monoclonal est produit chez la souris et reconnaît la séquence FLAG au niveau des extrémités N-terminale, Met N-terminale et C-terminale. Cet anticorps est également capable de reconnaître la séquence FLAG au niveau d'un site interne. Contrairement à l'anticorps M1, l'anticorps M2 n'est pas dépendant du calcium.
La référence F3165 est purifiée par affinité sur une résine avec de la protéine A : elle contient donc non seulement l'anticorps anti-FLAG M2 mais aussi de petites quantités d'IgG native de souris, ce qui augmente sa sensibilité dans la plupart des applications.
Méthode de purification - Protéine A

Immunogen

FLAG ; séquence peptidique DYKDDDDK

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Related product

Réf. du produit
Description
Tarif

F8775

Formaldéhyde solution, Molecular Biology, 36.5-38% in H2O

F1804

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

F9291

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse, clone M2, purified immunoglobulin, buffered aqueous glycerol solution

F7425

ANTI-FLAG® antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

H9658

Anticorps anti-HA monoclonal antibody produced in mouse, clone HA-7, ascites fluid

A2220

Gel d′affinité ANTI-FLAG® M2, purified immunoglobulin, buffered aqueous glycerol solution

P7582

Amino-terminal FLAG-BAP™ Fusion Protein

M4439

Anticorps monoclonal de souris anti-c-Myc antibody produced in mouse, clone 9E10, purified from hybridoma cell culture

CE0050

CelLytic™ MEM Protein Extraction Kit

Classe de stockage

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000512031
0000512031
0000493941
0000493938
0000493940

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Casey C Fowler et al.
Nature communications, 10(1), 3684-3684 (2019-08-17)
Bacterial toxins with an AB5 architecture consist of an active (A) subunit inserted into a ring-like platform comprised of five delivery (B) subunits. Salmonella Typhi, the cause of typhoid fever, produces an unusual A2B5 toxin known as typhoid toxin. Here
Fangzhi Tan et al.
Nature communications, 10(1), 3733-3733 (2019-08-21)
Hearing loss is the most common sensory disorder. While gene therapy has emerged as a promising treatment of inherited diseases like hearing loss, it is dependent on the identification of gene delivery vectors. Adeno-associated virus (AAV) vector-mediated gene therapy has
Hong Zhu et al.
Molecular biology of the cell, 24(11), 1619-1637 (2013-04-12)
Charcot-Marie-Tooth (CMT) disease is an inherited neurological disorder. Mutations in the small integral membrane protein of the lysosome/late endosome (SIMPLE) account for the rare autosomal-dominant demyelination in CMT1C patients. Understanding the molecular basis of CMT1C pathogenesis is impeded, in part
T P Molitor et al.
Oncogenesis, 2, e48-e48 (2013-06-05)
The vaccinia-related kinases (VRKs) comprise a branch of the casein kinase family. VRK1, a ser/thr kinase with a nuclear localization, is the most well-studied paralog and has been described as a proproliferative protein. In lower eukaryotes, a loss of VRK1
Jeong Gu Kang et al.
Scientific reports, 9(1), 11960-11960 (2019-08-21)
Despite the increased interest in epigenetic research, its progress has been hampered by a lack of satisfactory tools to control epigenetic factors in specific genomic regions. Until now, many attempts to manipulate DNA methylation have been made using drugs but
Afficher tous les articles scientifiques apparentés

Articles

Anti-FLAG® M2 Magnetperlen

Vergleich von Elutionstechniken für die Proteinaufreinigung von FLAG® Tag Proteinen im kleinen Maßstab mittels anti-FLAG® M2 Magnetperlen.

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

Contenu apparenté

Interactions entre protéines et acides nucléiques

Réactifs pour interactions entre protéines et acides nucléiques, ressources permettant d'étudier les interactions protéines/ARN, protéines/ADN et protéines/protéines, et applications associées.

Protein & Nucleic Acid Interactions

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

Protein- & Nukleinsäure-Wechselwirkungen

Interaktionsreagenzien für Protein und Nukleinsäure und Ressourcen für die Untersuchung von Protein-RNA-, Protein-DNA- und Protein-Protein-Interaktionen und damit verbundene Anwendungen.

Interazioni tra proteine e acidi nucleici

Reagenti e risorse per lo studio delle interazioni tra proteine​ e acidi nucleici, come le interazioni proteina-RNA, proteina-DNA e proteina-proteina, e delle relative applicazioni.

Afficher tout
1 Review This action will navigate to reviews.

Questions

1–5 of 5 Questions  

  1. Is there a recommended dilution for capture (sandwich) based ELISA?

    1 answer

    1. Monoclonal ANTI-FLAG® M2 may be used in EIA procedures. Typically, a fusion protein containing a FLAG® peptide sequence is directly adsorbed (or otherwise presented) within the wells of a multiwell polystyrene plate. The Monoclonal ANTI-FLAG® M2 antibody may be diluted up to 1:50,000 for subsequent incubation within the plate wells. Detection may be accomplished using Anti-Mouse IgG-Peroxidase (Cat. No. A9044) or equivalent, diluted 1:10,000, followed by an appropriate substrate for visualization.

      Dilutions for use as the capture antibody in an ELISA have not been evaluated. We would recommend that the end-user optimize the protocol following the recommendations found here:
      https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/elisa/elisa-procedures#antigen_coating

      Helpful?

  2. What is the recommended dilution for conducting western blotting using this antibody?

    1 answer

    1. The recommended dilution for Western Blot is 10 µg/mL. Please see the link below to review additional information, including protocols:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/274/912/f3165dat-ms.pdf

      Helpful?

  3. What is the recommended dilution for conducting immunoprecipitation (IP) using the M2 antibody?

    1 answer

    1. The general starting recommendation for conducting immunoprecipitation (IP) using the F3165, anti-FLAG M2 antibody, is 10 μg/mL.

      Helpful?

  4. What is the difference between F1804 and F3165 product?

    1 answer

    1. Product F1804 and F3165 are both Monoclonal ANTI-FLAG M2 antibodies produced in mouse Clone M2, with the same starting material. The distinction between the products lies in their purification methods. F3165 is immunoglobulin purified, while F1804 is immunogen affinity purified. F1804, being more purified, is expected to exhibit reduced background and less non-specific staining compared to F3165. Both products are suitable for western blotting, immunofluorescence, and immunoprecipitation.

      Helpful?

  5. What type of light chain is present on the FLAG M2 antibody?

    1 answer

    1. Test has not been conducted to determine whether the light chains of the antibody F3165, Monoclonal ANTI-FLAG® M2 antibody produced in mouse, are kappa or lambda. However, based on customer feedback, it appears that the M2 monoclonal antibody has lambda light chains and not kappa. A probe with anti-kappa did not yield a signal, while anti-lambda produced a good signal.

      Helpful?

1 Review This action will navigate to reviews.
1 out of 1 (100%) reviewers recommend this product

Reviews

Active Filters

  1. New Jersey
    • Review 1
    • Votes 15
    5 out of 5 stars.

    Great Anti Flag primary

    Works well for our western blot analysis of flag tagged recombinant proteins

    Helpful?

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique