The product is essentially a salt-free preparation and should be reconstituted to a minimum concentration of 1 mg/ml with a saline solution containing 150 mM NaCl.
I4506
IgG from human serum
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Synonyme(s) :
IgG humaine
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10 MG
233.00 CHF
50 MG
778.00 CHF
100 MG
1 230.00 CHF
500 MG
1 800.00 CHF
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Laissez-nous vous aiderNom du produit
IgG from human serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
conjugate
unconjugated
grade
reagent grade
assay
≥95% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
composition
Protein, ≥90%
technique(s)
ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable
storage temp.
2-8°C
Quality Level
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Cet article | I5131 | I4381 | 56834 |
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form fibers |
| technique(s) ELISA: suitable, immunoelectrophoresis: suitable, dot immunobinding: suitable, western blot: suitable | technique(s) - | technique(s) ELISA: suitable | technique(s) radioimmunoassay: suitable |
| grade reagent grade | grade reagent grade | grade reagent grade | grade - |
| assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (GE) |
Application
L′IgG de sérum humain a été utilisée comme étalon à une concentration de 1,274 mg/ml dans une analyse par ELISA pour déterminer les réponses d′anticorps à Porphyromonas gingivalis et Prevotella intermedia.[1] Elle a été utilisée pour l′immunomarquage de cellules Vero, BHK et PtK2 à une concentration de 0,2 mg/ml.[2] L′IgG de sérum humain a été utilisée comme étalon dans une analyse par SDS-PAGE d′anticorps anti-LPS de Shigella.[3]
Biochem/physiol Actions
Le sous-type d′anticorps IgG constitue la plus abondante des immunoglobulines du système immunitaire. Les IgG sont sécrétées par les lymphocytes B et sont présentes dans le sang et les liquides extracellulaires ; elles protègent contre les infections provoquées par les bactéries, champignons et virus. Les IgG maternelles sont transmises au fœtus à travers le placenta, ce qui est essentiel à la défense immunitaire des nouveau-nés contre les infections.[4]
Disclaimer
Sauf indication contraire dans nos catalogues ou autre documentation de la Société accompagnant de(s) produit(s), nos produits sont conçus uniquement pour la recherche et ne peuvent pas être utilisés dans d′autres buts, ce qui inclut mais sans s′y limiter, les utilisations commerciales non autorisées, les utilisations de diagnostic in vitro, les usages thérapeutiques ex vivo ou in vivo et tout type de consommation par ou d′application à l′homme ou aux animaux.
General description
Les IgG humaines purifiées sont isolées à partir d'un pool de sérums humains normaux par fractionnement et chromatographie d'échange d'ions. Ce produit est fourni sous la forme d'une poudre lyophilisée quasiment exempte de sels (moins de 1 % de sodium).
Related product
Réf. du produit
Description
Tarif
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Molecular biology of the cell, 13(8), 2795-2809 (2002-08-16)
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Egil S Blix et al.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 21(5), 840-847 (2015-02-18)
Multiple myeloma (MM) is considered an incurable B cell malignancy, although many patients can benefit from high-dose therapy with autologous stem cell transplantation (ASCT) as a first-line treatment. In non-Hodgkin lymphoma (NHL), ASCT is usually performed after relapse with curative
Nak-Hyeon Kim et al.
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Surface plasmon resonance (SPR) sensors based on a silver film suffer from signal degradation due to silver oxidation in aqueous sensing environments. To overcome this limitation, we fabricated the planar plasmonic substrate employing an atomic MoS2 layer on a silver
Nils A Brechmann et al.
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High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the development of a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarified CHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum
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Datasheet
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How is this standard reconstituted?
1 answer-
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What is the expected MW of the product?
1 answer-
The molecular weight of this product is not determined on a lot to lot basis. According to published literature, the molecular weight of Immunoglobulin G (IgG) is approximately 150 - 160 KDa. Historical data available for this product indicates that in SDS-PAGE electrophoresis this material will have multiple bands ranging from 155 kDa - 220 kDa.
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Necesito saber la secuencia de la proteína o el número para buscarla en uniprot
1 answer-
The protein sequence is not determined for this product. Human IgG has 4 subclasses: IgG1, IgG2, IgG3 and IgG4. These are present in serum at approximately 66%, 23%, 7% and 4%, respectively.
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What is the isoelectric point of this
1 answer-
This product is not tested on a lot-to-lot basis to determine the isoelectric point. However, historical data indicates an isoelectric point range of 6.1 - 8.5.
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Is product I4506, because it is derived from human serum, considered to potential biohazard?
1 answer-
As indicated in the corresponding Data Sheet at the link below: "BIOHAZARD: Handle as if capable of transmitting infectious agents". The source material is tested and found negative for antibody to HIV, antibody to HCV, and for HbsAG.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdfHelpful?
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What is the recommended storage method for item I4506, IgG from human serum, after reconstitution?
1 answer-
After reconstitution, keep aliquots at 2-8°C for short-term use (up to 4 weeks) and store the remaining aliquots at -20°C.
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What is the significance of absorbance at 280 nm (E280 1% = 14)?
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The absorbance at 280 nm (E280 1% = 14) indicates the percent extinction coefficient (ϵ1%).
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what is the expected molecular weight under reducing conditions
1 answer-
I4506, Human IgG, is a tetrameric protein consisting of 2 light chains and 2 heavy. When run on a reducing gel, The heavy chain will be detectable at ~50kDa, and the light chain at ~25kda.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product I4506, IgG from human serum, tested for endotoxin?
1 answer-
No. Product No. I4506 is not tested for endotoxin levels.
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