The integrity of Spermidine can be verified by Gas chromatography. The following publication may be of interest for protocol reference on Gas chromatography analysis of Spermidine.
1. J Mass Spectrom. 2009 Aug;44(8):1203-10. doi: 10.1002/jms.1597.
2. A quantitative GC-MS method for three major polyamines in postmortem brain cortex, PMID: 19514045
S0266
Spermidine
BioReagent, Molecular Biology, suitable for cell culture, ≥98%
Synonyme(s) :
1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane
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1 G
67.40 CHF
5 G
364.00 CHF
25 G
819.00 CHF
67.40 CHF
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A propos de cet article
Formule linéaire :
NH2(CH2)3NH(CH2)4NH2
Numéro CAS:
Poids moléculaire :
145.25
Beilstein:
1698591
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352200
ID de substance PubChem :
Nomenclature NACRES :
NA.52
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Laissez-nous vous aiderQualité
Molecular Biology
Niveau de qualité
Gamme de produits
BioReagent
Essai
≥98%
Forme
solid or liquid
Poids mol.
145.25 (AMU)
Technique(s)
cell culture | mammalian: suitable
Indice de réfraction
n20/D 1.479 (lit.)
Densité
0.925 g/mL at 25 °C (lit.)
Activité étrangère
DNase, RNase, and protease, none detected
Température de stockage
room temp
Chaîne SMILES
NCCCCNCCCN
InChI
1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
Clé InChI
ATHGHQPFGPMSJY-UHFFFAOYSA-N
Informations sur le gène
rat ... Ppm1a(24666)
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1 of 4
Cet article | 85558 | S2626 | 49761 |
|---|---|---|---|
| assay ≥98% | assay ≥99.5% (GC) | assay ≥99% (GC) | assay ≥97.5% (NT), ≥99.0% (GC) |
| form solid or liquid | form - | form solid or liquid (clear) | form - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| technique(s) cell culture | mammalian: suitable | technique(s) ChIP: suitable, buffer exchange: suitable | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. 2-8°C |
| mol wt 145.25 (AMU) | mol wt - | mol wt - | mol wt - |
Description générale
Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.[1]
Application
Actions biochimiques/physiologiques
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T4 polynucleotide kinase activity.
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.
Clause de non-responsabilité
Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Eye Dam. 1 - Skin Corr. 1B
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
233.6 °F - closed cup
Point d'éclair (°C)
112 °C - closed cup
Équipement de protection individuelle
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Organization, developmental dynamics, and evolution of plastid nucleoids
Sato N, et al.
International Review of Cytology, 232, 217-262 (2003)
Spermidine delays aging in humans
Madeo F, et al.
Aging (Albany. NY.), 10(8), 2209-2209 (2018)
Elumalai Sivamani et al.
Plant cell reports, 28(2), 213-221 (2008-11-19)
We have developed a method by which remarkably higher efficiencies of transient and stable transformation were achieved in bombardment transformation of plants. Over fivefold increase in transient gus gene expression was achieved when rice or maize suspension cells were bombarded
Molecular Determinants of Metaplastic and Neoplastic Transformation in the Esophageal Epithelium
Progress in Molecular Biology and Translational Science, 96, 63-92 (2010)
Preclinical development of human granulocyte-macrophage colony-stimulating factor-transfected melanoma cell vaccine using established canine cell lines and normal dogs
Hogge GS, et al.
Cancer Gene Therapy, 6(1), 26-26 (1999)
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Can the reconstituted S0266 solution, which has been sitting at room temperature for 3 months, still be used in CUT&Tag assays to stabilize nuclei? Also, how can the integrity of spermidine be tested? If there's no more spermidine available to test the solution, should more be purchased?
1 answer-
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What is the solvent that spermidine is dissolved in?
1 answer-
This product is a neat or pure liquid and has not been diluted in a solvent. However, to further dilute this product, it is recommended to use water at 50 mg/mL water.
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How to store S0266-5G? which comes as liquid and what is the molaritty of it? which solvent to use to dilute it?
1 answer-
This product can be stored in room temperature in its original format. The concentration of this product can be determined using the lot specific purity reported on the Certificate of Analysis, the density, and the compound molecular weight. This product has a density of 0.925 g/mL and a molecular weight of 145.25 g/mol. The minimum purity is 98%, so the molarity will vary slightly from lot to lot. The molarity can range from 6.2M (98%) to 6.4M (100%).
Molarity=Density x 1000 x Purity/MWThis product is supplied as a liquid or solid. A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter.
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How to store S0266-1G which comes as liquid? alliquoted and store at -20C? How long it can be stored at this condition?
1 answer-
Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.
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How frequently should new solutions be made?
1 answer-
A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter. Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.
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Is this a spermidine solution or a neat liquid, and what is the concentration?
1 answer-
Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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