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Merck

SHC007V

MISSION® Luciferase shRNA Control Transduction Particles

shRNA sequence targeting luciferase

Synonyme(s) :

MISSION®, MISSION® Control Transduction Particles

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A propos de cet article

Code UNSPSC :
41106609
Nomenclature NACRES :
NA.51

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Gamme de produits

MISSION®

Concentration

≥1x106 VP/ml (via p24 assay)

Technique(s)

capture ELISA: 106 TU/mL using p24

Conditions d'expédition

dry ice

Température de stockage

−70°C

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Cet article
SHC005VSHC004VSHC002H
MISSION® Luciferase shRNA Control Transduction Particles shRNA sequence targeting luciferase

Sigma-Aldrich

SHC007V

MISSION® Luciferase shRNA Control Transduction Particles

MISSION® eGFP Control Transduction Particles shRNA sequence targeting eGFP

Sigma-Aldrich

SHC005V

MISSION® eGFP Control Transduction Particles

Particules de transduction MISSION® pLKO.1-puro TurboGFP™ pour le contrôle du petit ARN en épingle à cheveux (shRNA) shRNA sequence targeting tGFP

Sigma-Aldrich

SHC004V

Particules de transduction MISSION® pLKO.1-puro TurboGFP™ pour le contrôle du petit ARN en épingle à cheveux (shRNA)

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, High Titer Targets no known mammalian genes

Sigma-Aldrich

SHC002H

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, High Titer

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

concentration

≥1x106 VP/ml (via p24 assay)

concentration

≥1x106 VP/ml (via p24 assay)

concentration

≥1x106 VP/ml (via p24 assay)

concentration

≥1x109 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 106 TU/mL using p24

technique(s)

capture ELISA: 109 TU/mL using p24

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100, 200

Description générale

The MISSION Luciferase shRNA Control Transduction Particles contain an shRNA sequence targeting Luciferase, Photinus pyralis (GenBank Accession No. M15077). The Luciferase shRNA Control Particles are useful as a positive knockdown control in experiments using cell lines expressing firefly luciferase.
The Luciferase shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-luciferase shRNA (Prod. No. SHC007). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Informations légales

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
05021709MN

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

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