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ABC348

Anti-Bmf Antibody

Name: Anti-Bmf Antibody
Brand: MM

from rabbit, purified by affinity chromatography

Sinonimo/i:

Bcl-2-modifying factor, Bmf

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Informazioni su questo articolo

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC, WB

Citations:

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Proprietà

biological source

rabbit

Quality Segment

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable, western blot: suitable

UniProt accession no.

Q96LC9

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BMF(90427)

Descrizione

General description

Bcl-2-modifying factor (Bmf) is a member of the Bcl-2 family containing one BCL2 homology domain 3. Members of this family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell′s actin cytoskeleton. Bmf is constitutively expressed in many tissues. There are 3 isoforms of Bmf. Isoform 1 is mainly expressed in B-lymphoid cells while isoform 2 and 3 are mainly expressed in B-CLL and normal B-cells.

~25 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human Bmf.

Application

Detect Bcl-2-modifying factor using this rabbit polyclonal antibody, Anti-Bmf Antibody validated for use in western blotting & IHC.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Bmf in HeLa cells.

Biochem/physiol Actions

This antibody recognizes the N-terminus of Bmf.

Analysis Note

Control
HepG2 and HEK293 cell lysates

Evaluated by Western Blotting in HepG2 and HEK293 cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected Bmf in 15 µg of HepG2 and HEK293 cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Questo articolo	HPA010120	B1684	SAB1412330
Sigma-Aldrich ABC348 Anti-Bmf Antibody	Sigma-Aldrich HPA010120 Anti-BMF antibody produced in rabbit	Sigma-Aldrich B1684 Anti-Bmf, N-Terminal antibody produced in rabbit	Sigma-Aldrich SAB1412330 ANTI-BRAF antibody produced in mouse
description from rabbit, purified by affinity chromatography	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous solution	description clone 2F7, purified immunoglobulin, buffered aqueous solution
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 100	Quality Level 100	Quality Level 200	Quality Level 100
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified immunoglobulin
biological source rabbit	biological source rabbit	biological source rabbit	biological source mouse
technique(s) western blot: suitable, immunohistochemistry: suitable	technique(s) immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:200-1:500	technique(s) western blot: suitable	technique(s) indirect immunofluorescence: suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL
shipped in wet ice	shipped in wet ice	shipped in dry ice	shipped in dry ice

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Certificati d'analisi (COA)

Lot/Batch Number

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