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CB1024

Anti-ACTN4 Mouse mAb (4D10)

liquid, clone 4D10, Calbiochem®

Sinonimo/i:

Anti-Actinin α4

Autenticati per visualizzare i prezzi organizzativi e contrattuali.

Informazioni su questo articolo

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
4D10, monoclonal
Species reactivity:
mouse, human, rat
Application:
—
Citations:
-


biological source

mouse

Quality Segment

antibody form

purified antibody

antibody product type

primary antibodies

clone

4D10, monoclonal

form

liquid

does not contain

preservative

species reactivity

mouse, human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (20 µg/mL)
Paraffin Sections (3 µg/mL))

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTN4(81)

General description

Anti-ACTN4, mouse monoclonal, clone 4D10, recognizes the ~90-100 kDa ACTN4 in PC-12, HeLa, Raw 264.7, MCF-7, NIH3T3 cells & in human testes. It is validated for ELISA, WB, ICC, and paraffin sections.
Purified mouse monoclonal antibody. Recognizes the ~90-100 kDa ACTN4 protein.
Recognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.

Immunogen

A recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein

Application







ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (20 g/ml)
Paraffin Sections (3 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells
Positive Control
PC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Yamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg.39, 61.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

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WH0000081M1HPA001873SAB5600102
description

liquid, clone 4D10, Calbiochem®

description

clone 4D10, purified immunoglobulin, buffered aqueous solution

description

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

description

recombinant, expressed in HEK 293 cells, clone RM287, purified immunoglobulin

species reactivity

mouse, human, rat

species reactivity

mouse, rat, human

species reactivity

mouse, human, rat

species reactivity

human

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

-

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

Gene Information

human ... ACTN4(81)

Gene Information

human ... ACTN4(81)

Gene Information

human ... ACTN4(81)

Gene Information

human ... ACTN4(81)

clone

4D10, monoclonal

clone

4D10, monoclonal

clone

polyclonal

clone

recombinant monoclonal, RM287, monoclonal

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice


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