CC077

Human Collagen Type V

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

• Sinonimo/i: Collagen Type V
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75
• Biological source: human
• Form: solution
• Technique(s): cell culture | mammalian: suitable
• Concentration: 1 mg/mL
• Assay: 95% (SDS-PAGE)

Proprietà

• Nome del prodotto: Human Collagen Type V, 1 mg/mL, solution, BioReagent, suitable for cell culture
• biological source: human
• Quality Segment: 200
• assay: 95% (SDS-PAGE)
• form: solution
• manufacturer/tradename: Chemicon^®
• concentration: 1 mg/mL
• technique(s): cell culture | mammalian: suitable
• impurities: <0.5% non-collagen proteins, <1% collagen type III, <2% collagen type I, <2% collagen type IV
• input: sample type mesenchymal stem cell(s); sample type induced pluripotent stem cell(s); sample type pancreatic stem cell(s); sample type epithelial cells; sample type neural stem cell(s); sample type hematopoietic stem cell(s); sample type: human embryonic stem cell(s)
• NCBI accession no.: NM_000093.3
• UniProt accession no.: P20908
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... COL5A1(1289), COL5A2(1290)

Descrizione

General description

Collagen type V is fibril-forming collagen that is located in the lung, bone, and fetal membranes along with type I collagen. It is minor collagen present in the extracellular matrix.

Application

Human Collagen Type V has been used to study the interaction of high endothelial venule protein (hevin) with human collagen V by fluorescence polarization (FP) assay and surface plasmon resonance(SPR). It has also been used to incubate the 96 well plate to determine the protein levels in media and cell lysates of fibroblasts.

Biochem/physiol Actions

Collagen type V acts as a regulator of collagen fibrillogenesis in the cornea and skin dermis. It interacts with matrix collagens and structural proteins thereby contributing to the structural integrity of tissues. Lower levels of type V collagen lead to loss of corneal transparency and Ehler Danlos syndrome.

Physical form

Liquid, in 0.1M acetic acid, pH 3.0. No preservatives added.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze.

Purified by serial salt precipitations of a pepsin extraction of human fetal membranes and chromatography on DEAE-cellulose.

Other Notes

Molecular composition: α1(V)]2 α2(V), native triple helix. Purity and retention of the native helical structure were monitored by SDS-PAGE, ORD measurement, and by reaction with anti-collagen type-specific monoclonal antibodies. Product Source: Human placenta, negative for HBsAg, HCV and HIV 1&2 antibodies.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Informazioni sulla sicurezza

• Classe di stoccaggio: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable