Documenti fondamentali

Visualizza tutta la documentazione

Vai a

C-22010

Endothelial Cell Growth Medium

Name: Endothelial Cell Growth Medium
Brand: SIGMA

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

Sinonimo/i:

Primary Cell Culture Media

Autenticati per visualizzare i prezzi organizzativi e contrattuali.

Scegli un formato

Cambia visualizzazione

Voi/SKU	Disponibilità	Prezzo

Informazioni su questo articolo

NACRES:

NA.71

UNSPSC Code:

12352207

Prezzi e disponibilità al momento non sono disponibili

Servizio Tecnico

Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.

Permettici di aiutarti

Proprietà

form

liquid

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

Descrizione

General description

Endothelial Cell Growth Medium (Ready-to-use)

Application

Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.

Packaging

Part of a Cell Culture System offered in a variety of formats:
Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
Growth Medium Kit: Includes Basal Medium and SupplementPack
SupplementMix: Contains all media supplements premixed in one vial
SupplementPack: Contains all media supplements as individual vials

Preparation Note

Click here for more information.

Final supplement concentrations after addition to the basal medium:
Fetal Calf Serum: 0.02 ml / ml
Endothelial Cell Growth Supplement: 0.004 ml / ml
Epidermal Growth Factor (recombinant human): 0.1 ng / ml
Basic Fibroblast Growth Factor (recombinant human): 1 ng / ml
Heparin: 90 μg / ml
Hydrocortisone: 1 μg / ml

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Analysis Note

All lots of PromoCell Endothelial Cell Media are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Confronta articoli simili

Visualizza il confronto per intero

Mostra le differenze

1 of 1

Questo articolo	C22215	C22216	C22121
Sigma-Aldrich C22010 Endothelial Cell Growth Medium	Sigma-Aldrich C22215 Endothelial Cell Growth Medium	Sigma-Aldrich C22216 Endothelial Cell Growth Medium 2	Sigma-Aldrich C22121 Endothelial Cell Growth Medium MV 2
description Ready-to-use kit including Basal Medium and SupplementMix, 500 ml	description Basal Medium, phenol red-free, 500 ml	description Basal Medium, phenol red-free, 500 ml	description Kit including Basal Medium and SupplementPack, 500 ml
form liquid	form aqueous solution	form aqueous solution	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
packaging pkg of 500 ml	packaging pkg of 500 ml	packaging pkg of 500 ml	packaging pkg of 500 ml

Still not finding the right product?

Explore all of our products under Endothelial Cell Growth Medium

Classe di stoccaggio

10 - Combustible liquids

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documenti section.

Se ti serve aiuto, non esitare a contattarci Servizio Clienti

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.