A white polystyrene plate with a non-binding surface is recommended for this kit. Black plates have not been evaluated, but may have a lower signal. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/329/297/cs1040bul.pdf
CS1040
SIRT1 Assay Kit
sufficient for 100 assays
Sinonimo/i:
SIRT1 Activity Assay
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1 KIT
CHF 717.00
CHF 717.00
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Informazioni su questo articolo
Codice UNSPSC:
12161503
NACRES:
NA.84
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Servizio Tecnico
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Permettici di aiutartiimpiego
sufficient for 100 assays
Livello qualitativo
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... SIRT1(23411)
1 of 4
Questo articolo | CS0170 | 07-131 | 566322-M |
|---|---|---|---|
| usage sufficient for 100 assays | usage kit sufficient for 100 assays (1 mL) | usage - | usage - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| Gene Information human ... SIRT1(23411) | Gene Information human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112) | Gene Information human ... SIRT1(23411) | Gene Information - |
Descrizione generale
The SIRT1 Assay Kit offers all the reagents required for the measurement of purified SIRT1 activity and for screening of inhibitors/activators.
The assay procedure is based on a two-step enzymatic reaction. The first step is deacetylation by SIRT1 of a substrate that contains an acetylated lysine side chain. The second step is the cleavage of the deacetylated substrate by the Developing Solution and the release of a highly fluorescent group. The measured fluorescence is directly proportional to the deacetylation activity of the enzyme in the sample.
The assay procedure is based on a two-step enzymatic reaction. The first step is deacetylation by SIRT1 of a substrate that contains an acetylated lysine side chain. The second step is the cleavage of the deacetylated substrate by the Developing Solution and the release of a highly fluorescent group. The measured fluorescence is directly proportional to the deacetylation activity of the enzyme in the sample.
Applicazioni
Azioni biochim/fisiol
Sirtuins (Sir2) are an evolutionarily conserved family of NAD+ dependent histone/protein deacetylases that tightly couple the cleavage of NAD+ and the deacetylation of protein substrates. The reaction products are nicotinamide, the deacetylated product, and a novel metabolite, 2′-O-acetyl-ADP-ribose. The proteins within this family are named after the first protein discovered from this family, Sir2 (Silent Information Regulator 2). Besides gene silencing, sirtuin proteins are important in other processes such as cell cycling regulation and fatty acid metabolism. SIRT1 is the human homolog of Sir2 and the one most studied to date. SIRT1 mediates p53 dependent process, transcription regulation, muscle differentiation, adipogenesis, and protection from axonal degeneration. SIRT1 also participates in early embryogenesis, neurogenesis, and cardiogenesis.
Caratteristiche e vantaggi
The kit offers all the reagents required for the fast and easy measurement of purified SIRT1 activity and for screening of inhibitors/activators. Moreover, the kit contains an inhibitor (nicotinamide) and an activator (resveratrol) as negative and positive controls, respectively.
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
- SIRT1 Substrate (Fluorometric) 100 μL
- Developer Solution 1.5 mL
- S8446SIRT1 human, recombinant, expressed in E. coli, N-terminal histidine tagged, ≥90% (SDS-PAGE), buffered aqueous glycerol solution 150 μg
- NAD+ Solution 1 mL
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
188.6 °F
Punto d’infiammabilità (°C)
87 °C
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Hyperglycemia, like aging, induces chromatin remodeling in mouse hepatocytes in comparison to normoglycemia and younger age, respectively. Changes in glucose metabolism also affect the action and expression of sirtuins, promoting changes in chromatin conformation and dynamics. Here we investigate the
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Myocardial fibrosis contributes to cardiac dysfunction. Astaxanthin (AST), a member of the carotenoid family, is a well-known antioxidant, but its effect on and underlying mechanisms in myocardial fibrosis are poorly understood. In vivo, myocardial fibrosis and cardiac dysfunction were induced
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Contenuto correlato
Instructions
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Can I use black flat bottom plates with polystyrene, nonbonding surface in this measurment?
1 answer-
Helpful?
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CAN I USE THE SIRT1 ACTIVITY ASSAY KIT CS1040 TO ESTIMATE SIRT1 ENZYMATIC ACTIVITY IN MICE LUNG TISSUE LYSATE?
1 answer-
The kit is intended to measure purified SIRT1 activity and to screen the efficacy of inhibitors and activators. However, it has also been cited to assess SIRT1 enzymatic activity in cell lysates, including mice tissue lysates. The following citation may be of interest for reference:
Heliyon. 2024 Sep 4;10(17):e37083.
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