D4635

2′-Deoxycytidine 5′-triphosphate disodium salt

≥95%

• Sinonimo/i: dCTP-Na₂
• Formula empirica (notazione di Hill): C₉H₁₄N₃Na₂O₁₃P₃
• Numero CAS: 102783-51-7
• Peso molecolare: 511.12
• NACRES: NA.51
• PubChem Substance ID: 24893889
• UNSPSC Code: 41106305
• MDL number: MFCD00084683
• Assay: ≥95%
• Form: powder
• Storage temp.: −20°C

Proprietà

• Quality Level: 200
• assay: ≥95%
• form: powder
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
• InChI: 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
• InChI key: ABWVCNMFYVEBIB-CDNBRZBRSA-L

Descrizione

General description

2′-Deoxycytidine 5′-triphosphate (dCTP) is synthesized either by de novo pathway or from the multiple phosphorylation steps of cytidine. It is deaminated to deoxyuridine monophosphate (dUMP) which is convertible to deoxythymidine triphosphate (dTTP).

Application

2′-Deoxycytidine 5′-triphosphate disodium salt has been used:

• in polymerase chain reaction mix for the amplification of Alternaria (AM) toxin DNA[1]
• to spike oligonucleotide sample for capillary electrophoresis (CE) analysis[2]
• as a component in adenosine 5′-monophosphate (AMP) luciferase assay[3]

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Biochem/physiol Actions

2′-Deoxycytidine 5′-triphosphate (dCTP) is used to synthesize DNA by DNA polymerases or reverse transcriptases. dCTP is an allosteric regulator of deoxycytidylate (dCMP) deaminase.[4] It is used in studies of nucleotide pools, nucleotide selectivity and enzyme specificity studies, such as the preferred incorporation of dCTP by Rev1, a γ-family DNA polymerase.[5]

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable