D4635
2′-Deoxycytidine 5′-triphosphate disodium salt
≥95%
Sinonimo/i:
dCTP-Na2
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 10 mg | Per conoscere la disponibilità, visualizza il carrello | CHF 89.70 | |
| 25 mg | Per conoscere la disponibilità, visualizza il carrello | CHF 192.00 | |
| 100 mg | Per conoscere la disponibilità, visualizza il carrello | CHF 566.00 | |
| 1 g | Per conoscere la disponibilità, visualizza il carrello | CHF 2’710.00 |
Informazioni su questo articolo
Formula empirica (notazione di Hill):
C9H14N3Na2O13P3
Numero CAS:
Peso molecolare:
511.12
NACRES:
NA.51
PubChem Substance ID:
UNSPSC Code:
41106305
MDL number:
Assay:
≥95%
Form:
powder
Storage temp.:
−20°C
CHF 89.70
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutartiQuality Level
assay
≥95%
form
powder
shipped in
dry ice
storage temp.
−20°C
SMILES string
[Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
InChI
1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
InChI key
ABWVCNMFYVEBIB-CDNBRZBRSA-L
General description
2′-Deoxycytidine 5′-triphosphate (dCTP) is synthesized either by de novo pathway or from the multiple phosphorylation steps of cytidine. It is deaminated to deoxyuridine monophosphate (dUMP) which is convertible to deoxythymidine triphosphate (dTTP).
Application
2′-Deoxycytidine 5′-triphosphate disodium salt has been used:
- in polymerase chain reaction mix for the amplification of Alternaria (AM) toxin DNA[1]
- to spike oligonucleotide sample for capillary electrophoresis (CE) analysis[2]
- as a component in adenosine 5′-monophosphate (AMP) luciferase assay[3]
2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.
Biochem/physiol Actions
2′-Deoxycytidine 5′-triphosphate (dCTP) is used to synthesize DNA by DNA polymerases or reverse transcriptases. dCTP is an allosteric regulator of deoxycytidylate (dCMP) deaminase.[4] It is used in studies of nucleotide pools, nucleotide selectivity and enzyme specificity studies, such as the preferred incorporation of dCTP by Rev1, a γ-family DNA polymerase.[5]
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Questo articolo | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
| form powder | form liquid | form solution | form liquid |
| assay ≥95% | assay ≥99% | assay 99% (dNTP) | assay ≥99% |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Paola Ferraro et al.
Nucleic acids research, 38(6), e85-e85 (2009-12-17)
Eukaryotic cells contain a delicate balance of minute amounts of the four deoxyribonucleoside triphosphates (dNTPs), sufficient only for a few minutes of DNA replication. Both a deficiency and a surplus of a single dNTP may result in increased mutation rates
Xiaoli Xiang et al.
Analytical biochemistry, 460, 22-28 (2014-05-27)
A luciferase-based method was developed for measurement of 5'-adenylylsulfate (APS) reductase (APR), an enzyme of the reductive sulfate assimilation pathway in prokaryotes and plants. APR catalyzes the two-electron reduction of APS and forms sulfite and adenosine 5'-monophospahate (AMP). The luciferase-based
Genetics: Analysis of Genes and Genomes (2009)
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| D4635-25MG | 04061833006887 |
| D4635-1G | 04061826690567 |
| D4635-100MG | 04061833565575 |
| D4635-10MG | 04061833006870 |