E1645

Anti-Endothelin antibody produced in rabbit

whole antiserum

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00282240
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i), RIA
• Citations: 3

Proprietà

• biological source: rabbit
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• contains: 15 mM sodium azide
• species reactivity: human, rat, pig
• technique(s): indirect ELISA: 1:30,000, radioimmunoassay: 1:10,000 using ¹²⁵I-endothelin-1, human
• UniProt accession no.: P05305
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EDN1(1906) ; rat ... Edn1(24323)

Descrizione

General description

Endothelin consists of a family of potent vasoconstrictor peptides, which include four structurally related isoforms, ET-1, ET-2, ET-3 and vasointestinal contractor (VIC, β-ET).

Immunogen

synthetic human endothelin-1 conjugated to KLH

Application

Anti-Endothelin antibody produced in rabbit is suitable for immunodetection of endothelins (ET-1, ET-2, and ET-3) in various immunoassays including RIA (titer of 1:30,000) and ELISA (titer of 1:10,000). Antibodies that react specifically with endothelins (ET-1, ET-2, and ET-3) may be used to detect endothelins in tissue extracts and biological fluids as well as study differential tissue expression, and intracellular localization of the endothelin isoforms in the periphery and central nervous system.

Biochem/physiol Actions

Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. ETs can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. The biological actions of ETs are mediated by activation of phospholipase C through specific G protein-coupled receptors.

Reacts with endothelin-1 (ET-1), (human, porcine), endothelin-2 (ET-2), (human), and endothelin-3 (ET-3), (human, rat). Cross-reactivity is seen with sarafotoxin S6c, but not with big-endothelin 38 (human), big-endothelin 39 (rat) or atrial natriuretic peptide (human). There is minimal cross-reaction with angiotensin I (human).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.