The volume of the container is 2 mL. Reconstitution of this product should be performed using 0.2 µm filtered 20 mM Tris, pH 7.0, to prepare a stock solution of >= 25 ug/mL. This stock solution may be diluted immediately before use to achieve the final working concentration. Additional filtration is not recommended, as it may result in product loss due to adsorption onto the filter membrane.
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Informazioni su questo articolo
Nome del prodotto
hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤-0.6 ng/mL
mol wt
16.0 kDa
packaging
pkg of 4X25 μg, pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg
color
white to faint yellow cast
solubility
water: soluble 0.025 mg, clear, colorless to faintly yellow
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... FGF2 (2247)
General description
Application
Physical form
Analysis Note
1 of 1
Questo articolo | |||
|---|---|---|---|
| description FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture | description FGF-Basic, from human, recombinant, expressed in E. coli, carrier free | description recombinant, expressed in insect cells, ≥85% (SDS-PAGE) | description The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells. |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in insect cells | recombinant - |
| biological source human | biological source human | biological source human | biological source human |
| assay ≥97% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay ≥85% (SDS-PAGE) | assay >95% (SDS-PAGE) |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) cell culture | stem cell: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 100 |
| form lyophilized powder | form lyophilized powder | form frozen liquid | form - |
Classe di stoccaggio
11 - Combustible Solids
WGK
WGK 3
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
DPI (dispositivi di protezione individuale)
Eyeshields, Gloves, type N95 (US)
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What is the volume of the vial and how do I reconstitute the powder?
1 answer-
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Hey! I am using this product to prepare a stem cell media. I know the concentration is 0.025mg but it has two data sheets, one that its soluble in water (0.250ml) and other that says to dissolve in 20mM Tris Base pH 7.0. How many volume should I add?
1 answer-
The solubility of this product is tested in water. It is lyophilized from a solution containing 20 mM Tris and 1M NaCl with 50 ug BSA as a stabilizer. The product data sheet recommends reconstituting in the 20 mM Tris for optimum stability and long term storage.
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