No. F4680 is not recommended for mounting Oil Red O stained slides. Mounting medias suitable for use with Oil Red O are glycerol gelatin, Kaiser's Glycerol Gelatin(e) and glycerol or glycerol diluted with PBS.
There is a glycerol gelatin available that is suitable for mounting Oil Red O slides. The catalog number is GG1. To use either glycerol gelatin or Kaiser's glycerol gelatin(e) the bottle must be warmed to approximately 60°C before use. The mounting media is sold at room temperature and returns to a solid at approximately 37°C
F4680
Mezzo di montaggio acquoso Fluoromount™
liquid
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
25 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 164.00 |
Informazioni su questo articolo
UNSPSC Code:
12171500
NACRES:
NA.47
Servizio Tecnico
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Permettici di aiutartiNome del prodotto
Mezzo di montaggio acquoso Fluoromount™, for use with fluorescent dye-stained tissues
Quality Segment
form
liquid
technique(s)
microbe id | staining: suitable
color
clear
suitability
suitable for fluorescence, suitable for immunohistochemistry
application(s)
diagnostic assay manufacturing
hematology
histology
General description
Fluoromount è un mezzo di montaggio a base acquosa, simile a Gel Mountä, da impiegare con vetrini coprioggetti per la conservazione di tessuti colorati con coloranti fluorescenti. Questo prodotto è compatibile con marcature con isotiocianato di fluoresceina (FITC), ficoeritrina, ficocianina e alloficocianina. Può anche essere usato con altri marcatori fluorescenti come il TexasRedÒ e la rodamina. Dopo l′uso, il mezzo può essere rimosso con facilitò immergendo i vetrini in acqua deionizzata.
Application
Il mezzo di montaggio acquoso Fluoromount™ è stato usato per il montaggio di:
- vetrini coprioggetto su vetrini per esame microscopico in immunofluorescenza di cellule PC12 e HeLa
- cellule neuronali dell′ippocampo per studi con microscopio confocale
- sezioni di cervello per colorazione di immunofluorescenza
Legal Information
Fluoromount is a trademark of Diagnostic BioSystems, Inc.
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Questo articolo | |||
|---|---|---|---|
| description for use with fluorescent dye-stained tissues | description tissue mounting medium | description This Fluorescent Mounting Media, Aqueous is validated for use in Immunofluorescence for the detection of Fluorescent Mounting Media Aqueous. | description - |
| technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - | technique(s) - |
| suitability suitable for immunohistochemistry, suitable for fluorescence | suitability - | suitability - | suitability - |
| color clear | color - | color - | color - |
| application(s) diagnostic assay manufacturing | application(s) hematology | application(s) - | application(s) hematology |
| form liquid | form liquid | form liquid | form - |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 100 |
Classe di stoccaggio
10 - Combustible liquids
Che succede?
WGK 3
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
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Can this product be used for mounting Oil Red O stained slides?
1 answer-
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Can you explain why it's recommended not to refrigerate it? Does that still apply even after opening?
1 answer-
This product is provided as a clear, viscous solution. Refrigeration may cause it to become difficult to use for coverslipping. Its product Information states, "Do not refrigerate". This applies to storage after the product has been opened.
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What are some examples of aqueous mounting media without glycerol?
1 answer-
For use with fluorescence:
1. F4680 Fluoromount
2. F6182 FluoroshieldAqueous Mounting media with no glycerol and antifade agents:
1. 10981 Polyvinyl alcohol with DABCO
2. F6937 Fluoroshield with DABCO
3. F6812 Fluoroshield with PPD (not suitable for a select number of fluorescent dyes)Aqueous Mounting Media with no glycerol and another dye:
1. F6057 Fluoroshield with DAPI
2. F5932 Fluoroshield with PIAqueous Mounting Media suitable for use with light microscopy:
1.GG1 Glycerol gelatin
2.C9368 CC/MountHelpful?
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Is F4680 suitable for use as a mounting medium for AEC red IHC mounting?
1 answer-
Fluoromount is not intended for use with bright field microscopy or for coverslipping slides stained with AEC. Most fluorescent mounting media are somewhat alkaline, with a pH around 9, while AEC is typically formulated with an acetate buffer around pH 5.2. The difference in pH levels suggests that the AEC may fade quickly, especially if the slides are counterstained with a nuclear counterstain such as Mayer's Hematoxylin, which would also likely disappear rapidly.
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Does this (Fluoromount F4680) contain DAPI?
1 answer-
This product does not contain DAPI. Should DAPI be preferred, see product F6057.
F4680 Fluoromount datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/692/371/f4680pis.pdfF6057 Fluoroshield with DAPI product page:
https://www.sigmaaldrich.com/product/sigma/f6057Helpful?
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Does Fluoromount mounting media (Product No. F4680) contain an antifade reagent?
1 answer-
Fluoromount does not contain an antifade reagent. If you need an antifade mounting media, check Fluoroshield (Product Nos. F6182, F6057 and F5932) or PVA with antifade (Product Nos. 10981 and 10979).
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How do you remove the coverslip after using Fluoromount (Product No. F4680)?
1 answer-
Place the slide in a beaker full of deionized water on a magnetic stirrer. Gently stir the slide for a few hours to overnight for complete removal of dry Fluoromount.
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Does Fluoromount mounting media (Product No. F4680) contain glycerol?
1 answer-
Fluoromount does not contain glycerol. The exact formulation is considered proprietary.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does Product F4680, Fluoromount Mounting Medium, compare to GelMount (Product No. G0918) which has been deleted?
1 answer-
Product No. F4608, Fluoromount is essential the same as GelMount. GelMount was deleted by the supplier. Fluoromount is being made by another supplier with the same formulation.
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