M2003

MTT Formazan

powder

• Sinonimo/i: Thiazolyl blue formazan
• Formula empirica (notazione di Hill): C₁₈H₁₇N₅S
• Numero CAS: 57360-69-7
• Peso molecolare: 335.43
• NACRES: NA.47
• PubChem Substance ID: 24896591
• UNSPSC Code: 12171500
• MDL number: MFCD00080731

Proprietà

• assay: ≥97% (TLC)
• Quality Segment: 200
• form: powder
• color: purple
• solubility: acetone: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
• InChI: 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
• InChI key: GIFKVQOPAJDJLO-SZVLODPRSA-N

Descrizione

Application

MTT Formazan has been used to measure cell viability.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• Che succede?: WGK 3
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable
• DPI (dispositivi di protezione individuale): Eyeshields, Gloves, type N95 (US)