OGS140

PSF-CMV-COOH-EKT-GST3 - C-TERMINAL GST PLASMID

plasmid vector for molecular cloning

• Sinonimo/i: cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• NACRES: NA.85
• UNSPSC Code: 12352200
• Promoter: Promoter name: CMV; Promoter activity: constitutive; Promoter type: mammalian
• Origin of replication: pUC (500 copies)
• Bacteria selection: kanamycin
• Reporter gene: none
• Peptide cleavage: EKT

Proprietà

• tag: GST tagged
• form: buffered aqueous solution
• mol wt: size 4900 bp
• bacteria selection: kanamycin
• origin of replication: pUC (500 copies)
• peptide cleavage: EKT
• peptide tag location: C-terminal
• promoter: Promoter name: CMV; Promoter activity: constitutive; Promoter type: mammalian
• reporter gene: none
• shipped in: ambient
• storage temp.: −20°C

Descrizione

General description

This vector adds a GST tag positioned at the 3 prime end (C-terminus) of a gene (expressed protein) that is within the primary MCS (NotI to XbaI). The tag can be used to purify a protein by binding to glutathione and the tag can also be used to increase the solubility of recombinant protein produced in bacterial cells. For reference the coding sequence of the GST tag is one base pair out of frame with the TAG stop codon that is found within the upstream XbaI site. There is an enterokinase cleavage site immediately upstream of this tag (DDDDK) that can be used to remove the tag from a purified protein if required.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Application

Cloning in a gene: There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI. There are built in Shine-Dalgarno and KOZAK sequences aligned with the start codon within the NcoI restriction site. If these are removed you will need to add a ribosomal entry site to the 5 prime end of your gene for optimal expression.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

Other Notes

Quick-reference Plasmid Map

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics^™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Genebank Vector Sequence File

FASTA Vector Sequence File

Full Plasmid Map

Legal Information

Oxford Genetics is a trademark of Oxford Genetics Ltd

Informazioni sulla sicurezza

• Classe di stoccaggio: 12 - Non Combustible Liquids
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable