UPBST-RT-HC

Ultra pure BST-RT Polymerase

Suitable for further manufacturing

• Sinonimo/i: Polymerase, Reverse Transcriptase
• UNSPSC Code: 12352204
• Recombinant: expressed in E. coli (Expressed in E.coli (Recombinant))
• Concentration: 50-350 mM (optimal salt concentration)

Proprietà

• recombinant: expressed in E. coli (Expressed in E.coli (Recombinant))
• Quality Segment: 400
• grade: Molecular Biology
• sterility: sterile; 0.45 μm filtered
• form: liquid
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 50-350 mM (optimal salt concentration)
• technique(s): RT-qPCR: suitable (Isothermal amplification), cDNA synthesis: suitable
• impurities: (DNase free, RNasefree, DNA free, NICKase free)
• color: colorless
• suitability: suitable for PCR (Isothermal amplification)
• shipped in: dry ice
• storage temp.: −20°C (−15°C to −25°C)

Descrizione

General description

BST-RT Polymerase is an engineered Bst enzyme with robust reverse transcriptase (RT) activity, making it ideal for use in loop-mediated isothermal amplification (LAMP) or RT-LAMP assays. This feature eliminates the necessity of a secondary RT enzyme when targeting amplification from RNA samples, simplifying reaction setup. BST-RT works optimally at 65°C and can be used for real time reaction monitoring with a fluorescent dye or in endpoint detection assays. BST-RT polymerase is tolerant to potential reaction inhibitors such as ethanol, extraction buffers, and saliva. This enzyme is also compatible with workflows that incorporate dUTP for elimination of reaction carryover contaminants.

Application

Loop-mediated isothermal amplification (LAMP) has emerged as a key alternative to polymerase chain reaction (PCR)-based techniques for amplifying nucleic acids. While conventional PCR employs a thermal cycler for repetitive cycling temperatures when amplifying, isothermal amplification techniques occur at a single and fixed temperature, allowing the use of simple,portable, and more robust instruments for fast and exponential amplification.

Features and Benefits

• Compatible with samples containing either DNA or RNA
• Ability to perform RT-LAMP without additional RT enzymes
• Lacks 5′ to 3′ exonuclease domain
• DNase free, RNase free, DNA free, and Nickase free
• Active from 25-65 °C (optimal at 65 °C)
• Unit Definition: One unit is the amount of enzyme required to incorporate 10 nmol of dNTP onto ssDNA template for 30minutes at 65°C
• Also available in stand-alone, high concentrations and glycerol-free formulations

Preparation Note

In solution.

Other Notes

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C. Order primers for LAMP using our online configurator. For assistance with primer design prior to purchase, please use the online design tool from our partner.

Informazioni sulla sicurezza

• Classe di stoccaggio: 10 - Combustible liquids
• Che succede?: WGK 2
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable