This product has not been tested for any specific application, so a specific protocol is not available. However, this material has been used successfully by a number of researchers.
Please see the link below to review publications that may be helpful:
https://www.sigmaaldrich.com/search/287810?focus=papers&page=1&perpage=30&sort=relevance&term=287810&type=citation_search
287810
2′,7′-Dichlorofluorescin Diacetate
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.
Synonyma:
2′,7′-Dichlorofluorescin Diacetate, H2DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate
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| Vám/SKU | Dostupnost | Cena |
|---|---|---|
100 mg | Odhaduje se, že bude odesláno dnesodAreál Kühne+Nagel spol. s r.o. | 2 740,00 Kč |
O této položce
Empirický vzorec (Hillův zápis):
C24H16Cl2O7
Číslo CAS:
Molekulová hmotnost:
487.29
UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:
Assay:
≥94% (HPLC)
Form:
crystalline solid
Storage condition:
OK to freeze, protect from light
2 740,00 Kč
Odhaduje se, že bude odesláno dnespodrobné informace
Technický servis
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Dovolte nám, abychom vám pomohliQuality Segment
assay
≥94% (HPLC)
form
crystalline solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
technique(s)
protein detection: suitable
solubility
DMSO: 5 mg/mL
shipped in
ambient
storage temp.
−20°C
SMILES string
Clc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)Cl)C2c4c(cccc4)C(=O)O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChI key
PXEZTIWVRVSYOK-UHFFFAOYSA-N
General description
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and the nitric oxide (NO) in neutrophils and macrophages and for the determination of the overall oxidative stress in toxicological phenomena.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Detection of reactive oxygen species (ROS) and nitric oxide (•NO)
Detection of reactive oxygen species (ROS) and nitric oxide (•NO)
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Crow, J.P. 1997. Nitric Oxide 1, 145.
Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta1291, 245.
Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.
Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta1291, 245.
Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Tato položka | |||
|---|---|---|---|
| description Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress. | description ≥97% | description BioReagent, suitable for fluorescence, ≥85% (HPCE) | description BioReagent, suitable for fluorescence, ≥90% (T) |
| assay ≥94% (HPLC) | assay ≥97% | assay ≥85% (HPCE) | assay ≥90% (T) |
| form crystalline solid | form powder | form - | form solid |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
| storage condition protect from light, OK to freeze | storage condition - | storage condition - | storage condition - |
Skladovací třída
11 - Combustible Solids
Co se děje?
WGK 3
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