This product is recommended for use in fluorescence microscopy and has not been evaluated for use in Flow Cytometry. However, the following citation reports the use of BioTracker 488 Green Lipid Droplet Dye in Flow Cytometry.
SCT144
BioTracker 488 Green Lipid Droplet Dye
Live cell imaging dye that rapidly stains lipid droplets with no wash steps and minimal background staining of other cellular membranes or organelles.
Synonyma:
Live cell imaging probe, Live cell lipid imaging probe
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Vybrat velikost
Změnit zobrazení
| Vám/SKU | Dostupnost | Cena |
|---|---|---|
125 μL | Odhaduje se, že bude odesláno dnesodAreál Kühne+Nagel spol. s r.o. | 12 900,00 Kč |
O této položce
NACRES:
NA.47
UNSPSC Code:
12352207
12 900,00 Kč
Odhaduje se, že bude odesláno dnespodrobné informace
Technický servis
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cell based assay: suitable, live cell imaging : suitable
detection method
fluorometric
shipped in
ambient
storage temp.
2-8°C
General description
Lipids are fundamental building blocks of cells and play important cellular roles. Lipids are key components of the plasma membrane and other cellular compartments, including the nuclear membrane, endoplasmic reticulum, Golgi apparatus, and trafficking vesicles such as endosomes and lysosomes. Intracellular lipid droplets are cytoplasmic organelles involved in the storage and regulation of triglycerides and cholesterol esters. Lipids have been implicated in various diseases including heart, vascular and neurological diseases.
BioTracker Lipid dyes rapidly stain lipid droplets in live or fixed cells with no wash step and minimal background staining of cellular membranes or other organelles. Cells also can be fixed and permeabilized after staining.
The BioTracker 488 Green Lipid Dye has an excitation around 430 nm, and can be excited equally well at 405 nm or 488 nm. In cells, it stains lipid droplets with bright green fluorescence using the FITC detection channel.
Spectral Properties
Absorbance: 427nm
Emission: 585nm
BioTracker Lipid dyes rapidly stain lipid droplets in live or fixed cells with no wash step and minimal background staining of cellular membranes or other organelles. Cells also can be fixed and permeabilized after staining.
The BioTracker 488 Green Lipid Dye has an excitation around 430 nm, and can be excited equally well at 405 nm or 488 nm. In cells, it stains lipid droplets with bright green fluorescence using the FITC detection channel.
Spectral Properties
Absorbance: 427nm
Emission: 585nm
Application
Live cell fluorescent imaging
Live cell imaging dye that rapidly stains lipid droplets with no wash steps and minimal background staining of other cellular membranes or organelles.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Physical form
Liquid, supplied as a 1000X solution in DMSO
Preparation Note
Store BioTracker 488 Green Lipid Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Spectral Properties
Absorbance: 427nm
Emission: 585nm
Absorbance: 427nm
Emission: 585nm
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tato položka | |||
|---|---|---|---|
| description Live cell imaging dye that rapidly stains lipid droplets with no wash steps and minimal background staining of other cellular membranes or organelles. | description Live cell imaging mitochondrial dye that stains the membrane of mitochondria used to detect cell viability, metabolic activity and overall cell health. | description Live cell imaging cell proliferation kit based on carboxyfluorescein succinimidyl ester (CFSE) used for fluorescent cell labeling in flow cytometry applications. | description Live cell imaging microtubule dye for imaging the cytoskeleton of living cells |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. -10 to -25°C | storage temp. -10 to -25°C | storage temp. 2-8°C |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
Skladovací třída
10 - Combustible liquids
Co se děje?
WGK 1
Bod vzplanutí (°F)
188.6 °F - (refers to pure substance)
Bod vzplanutí (°C)
87 °C - (refers to pure substance)
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Good afternoon, I would like to to know if it is possible to use the reagent also by flow cytometry application, and if there are any references or examples of it having been successfully used in this context, thank you
1 answer-
Helpful?
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Are there any solutions for the high background issue experienced when using the product with heart tissue cells?
1 answer-
The high background issue could be due to the use of antifade, which is not recommended. Instead, it's suggested to use PBS as the mounting medium and nail polish as the coverslip sealant. Additionally, using other mounting media such as EverBrite™ or FluoroShield™ may impact staining patterns, so it's important to consider the recommended mounting methods to avoid issues with signal intensity and staining patterns.
Helpful?
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Is BioTracker 488 Green Lipid Dye (SCT144) toxic for the cells? Can they be monitored for extra time after staining? How long does the stain last in this case?
1 answer-
No cytotoxicity of this dye has been observed with incubation periods of up to 24 hours. Cytotoxicity beyond the 24-hour incubation period has not been studied. Suitability for extended incubation times would have to be determined by the end user.
Helpful?
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