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KK4752

Roche

KAPA PROBE FAST One-Step

Name: KAPA PROBE FAST One-Step
Brand: ROCHE
Price: 7427.2 CZK

Universal

Synonyma:

RT-qPCR, probe qPCR, qPCR

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Vám/SKU	Dostupnost	Cena
500 μL	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	8 440,00 Kč 7 427,20 Kč

O této položce

NACRES:

NA.55

UNSPSC Code:

41106300

7 427,20 Kč

Ceníková cena8 440,00 KčUložit 12 %

Akce probíhá pouze na webových stránkách

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

Technický servis

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Vlastnosti

usage

sufficient for 500 reactions

feature

dNTPs included: no, hotstart: no

packaging

pkg of 500 RXNS

manufacturer/tradename

Roche

technique(s)

RT-qPCR: suitable, whole genome amplification: suitable

input

purified DNA

detection method

probe-based

storage temp.

−20°C

Popis

General description

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal Kit is a sensitive and convenient solution for real-time PCR using RNA as template. The kit is designed for high-throughput, fast-cycling, one-step RNA quantification using sequence-specific fluorogenic probes. It is compatible with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g. TaqMan^®) and displacement probes (e.g., molecular beacons)

Application

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal has been used for:

Gene expression analysis
SNP/mutation analysis
Gene knockdown validation
RT-PCR

Features and Benefits

Quick Notes:

The kit is suitable for all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan) and displacement probes (e.g., molecular beacons).
Use only gene-specific primers for one-step qRT-PCR.
Optimal cDNA synthesis is achieved at 42°C for 5 min.
3 min at 95°C is sufficient for RT inactivation and DNA polymerase activation.
Do not exceed 25 μL reaction volumes

Packaging

Kit includes qPCR Master Mix (2X), KAPA RT Mix (50X), dUTP (10 mM), ROX High Reference Dye (50X) and ROX Low Reference Dye (50X)

Preparation Note

Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA PROBE FAST qPCR Master Mix may not freeze solidly, even when stored at -20 °C. The KAPA RT Mix is temperature-sensitive, and should be stored at -20 °C and kept on ice during use. ROX reference dye is light-sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

Analysis Note

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

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Tato položka	PFLRKB	PFABIKB	PFORCEKB
Roche KK4752 KAPA PROBE FAST One-Step	Roche PFLRKB KAPA PROBE FAST	Roche PFABIKB KAPA PROBE FAST	Roche PFORCEKB KAPA PROBE FORCE
description Universal	description suitable for qPCR, 2 ×, ROX^™ Low	description suitable for qPCR, 2 ×, ABI Prism^®	description 2 ×, Universal
technique(s) whole genome amplification: suitable, RT-qPCR: suitable	technique(s) qPCR: suitable, whole genome amplification: suitable	technique(s) qPCR: suitable, whole genome amplification: suitable	technique(s) whole genome amplification: suitable, qPCR: suitable
usage sufficient for 500 reactions	usage sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 1000 reactions	usage -	usage 20 μL sufficient for 100 reactions, 20 μL sufficient for 500 reactions
feature dNTPs included: no, hotstart: no	feature hotstart, dNTPs included	feature -	feature hotstart, dNTPs included
input purified DNA	input purified DNA	input -	input crude DNA
detection method probe-based	detection method probe-based	detection method -	detection method probe-based
packaging pkg of 500 RXNS	packaging kit of 5 mL (500 x 20 μL rxn; KK4717), kit of 1 mL (100 x 20 μL rxn; KK4716), kit of 10 mL (1000 x 20 μL rxn; KK4718), pkg of	packaging kit of 1 mL (100 x 20 μL rxn; KK4705), kit of 50 mL (5000 x 20 mL rxn; KK4714), pkg of , kit of 5 mL (500 x 20 μL rxn; KK4706), kit of 10 mL (1000 x 20 μL rxn; KK4707)	packaging kit of 1 mL (100 x 20 μL rxn; KK4300), kit of 5 mL (500 x 20 μL rxn; KK4301)

Skladovací třída

12 - Non Combustible Liquids

Co se děje?

WGK 1

Bod vzplanutí (°F)

does not flash

Bod vzplanutí (°C)