95388

Bismuth Sulfite Agar

NutriSelect^® Basic, according to GB 4789.4-2016, powder, selective for Salmonella spp., pack of 500 g, suitable for microbiology

• Synonyma: Salmonella Selective Agar
• NACRES: NA.74
• UNSPSC Code: 41171606
• eCl@ss: 32160607
• Manufacturer/tradename: NutriSelect^® Basic
• Agency: according to GB 4789.4-2016
• Form: powder
• Suitability: selective for Salmonella spp.
• Application(s): clinical testing; environmental; food and beverages, microbiology

Vlastnosti

• agency: according to GB 4789.4-2016
• Quality Segment: 100
• sterility: non-sterile
• product line: NutriSelect^® Basic
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L , bismuth ammonium citrate, 1.85 g/L , brilliant green, 0.025 g/L , disodium hydrogen phosphate, 4 g/L , D(+)-glucose, 5 g/L , iron(II) sulfate 7x H₂O, 0.55 g/L (equivalentto 0.3g/L iron(ll) sulfate water free), meat extract, 5 g/L , mixed peptone, 10 g/L , sodium sulfite, 6.15 g/L
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• color: greenish yellow
• final pH: 7.6±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages, microbiology
• suitability: selective for Salmonella spp.

Popis

General description

Bismuth Sulfite Agar is a highly selective medium designed for the isolation and detection of Salmonella species, including Salmonella typhi, from food, water, and clinical samples. The medium′s selectivity is achieved through the combined action of freshly precipitated bismuth sulfite and brilliant green, which effectively suppresses the growth of coliforms while permitting the growth of salmonellae. Sulfur compounds in the medium provide a substrate for hydrogen sulfide production, and the metallic salts, stain the colonies and surrounding medium black or brown in the presence of hydrogen sulfide, providing a clear visual indicator for Salmonella identification.

Application

Bismuth Sulfite Agar is used for the selective isolation and detection of Salmonella species in food, water, and clinical specimens. The medium is particularly valuable in food safety and clinical microbiology laboratories where the detection of Salmonella is critical. When the sample contains high levels of organic matter, it is recommended to suspend the sample in sterile saline and use the supernatant for inoculation to prevent the formation of atypical colonies.

Features and Benefits

NutriSelect^® Basic is a powdered culture media offering

• Quality control with growth promotion test according to industry standards
• Quality assurance acc. to ISO 9001
• Cost-efficient powdered media

Preparation Note

Dissolve 47.55 g in litre distilled water and heat to boiling to dissolve the medium completely. DO NOT STERILIZE. This medium should be prepared, and the plates poured, 24h in advance of their intended use, since the freshly prepared medium is excessively toxic to Salmonellae. It must also be remembered the medium loses selectivity after 3-4 days, even when refrigerated.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product will be discontinued in the near future; however, stock is still available. The recommended replacement product is 1.00191 - Bismuth Sulfite (BS) Agar

Bezpečnostní informace

• Kódy nebezpečí: H412
• Bezpečnostní pokyny: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• supp_hazards: EUH031
• Skladovací třída: 11 - Combustible Solids
• WGK: WGK 3
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)