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A2576

Agarose

Ultra-low Gelling Temperature, molecular biology grade

Synonyma:

3,6-Anhydro-α-L-galacto-β-D-galactan

Přihlásit pro zobrazení organizačních a smluvních cen.

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Vám/SKUDostupnostCena
5 g
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7 200,00 Kč
25 g
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23 000,00 Kč

O této položce

Číslo CAS:
UNSPSC Code:
41105317
PubChem Substance ID:
EC Number:
232-731-8
NACRES:
NA.21
MDL number:
eCl@ss:
32110690

7 200,00 Kč


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grade

Molecular Biology

Quality Segment

type

Type IX-A

form

powder

technique(s)

electrophoresis: suitable

impurities

≤7% water

ash

≤0.4%

mp

≤62 °C

transition temp

gel point ≤20 °C (1.5% gel)

gel strength

≥300 g/cm2 (1.5% gel)

anion traces

sulfate (SO42-): ≤0.14%

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

General description

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible and possesses variable mechanical and diffusion properties. Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically.

Application

Agarose is super low melting point and recommended for use in capillary electrophoresis, tissues & cell culture, and viral plaque assay.
Yields unusually strong gels for an ultra-low gelling agarose. Ideal for electrophoresis of heat-labile samples and for growth of hybridomas or other cell lines.
Agarose has been used:
  • in thaumatin and elastase protein crystallization
  • in microcasting using a polydimethylsiloxane (PDMS) mold with micro-channel
  • in agarose micro-confinement array

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Other Notes

Electroendosmosis: ≤0.12
Discover LiChropur reagents ideal for HPLC or LC-MS analysis

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Tato položka
A5030A0169121853
Agarose Ultra-low Gelling Temperature, molecular biology grade

Sigma-Aldrich

A2576

Agarose

Agarose Ultra-low Gelling Temperature

Sigma-Aldrich

A5030

Agarose

Agarose Type I-A, low EEO

Sigma-Aldrich

A0169

Agarose

Agarose Type I, Molecular Biology

Millipore

121853

Agarose

description

Ultra-low Gelling Temperature, molecular biology grade

description

Ultra-low Gelling Temperature

description

Type I-A, low EEO

description

Type I, Molecular Biology

form

powder

form

powder

form

powder

form

-

EEO

≤0.12

EEO

≤0.05

EEO

0.09-0.13

EEO

-

gel strength

≥300 g/cm2 (1.5% gel)

gel strength

-

gel strength

≥1200 g/cm2 (1% gel)

gel strength

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

grade

Molecular Biology

grade

-

grade

-

grade

Molecular Biology

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-


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Skladovací třída

11 - Combustible Solids

WGK

WGK 1

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

PPE (osobní ochranné prostředky)

Eyeshields, Gloves, type N95 (US)



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the molecular weight of this agarose?

    1 answer
    1. The molecular weight of this product has not been determined.

      Helpful?

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