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12106C

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

Synonyma:

HI FBS, HI FCS, HI Fetal Bovine Sera, HI Fetal Bovine Serum, FBS, FCS, sera, serum

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O této položce

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
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biological source

bovine fetus

Quality Segment

sterility

sterile-filtered

product line

FBS Premier

composition

hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Application

Fetal Bovine Serum (FBS) provides many non-defined growth promoting and survival enhancing factors to cells in culture. FBS, heat inactivated may be used in place of regular FBS wherein the inactivation of the complement system is beneficial to cell growth or function. Heat-inactivated FBS is used to culture Leishmania sp.

Preparation Note

Prepared by heating at 56°C for 30 minutes.
Prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes is used to inactivate the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions


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Tato položka
12003CF039212103C
description

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

description

Australia origin, USDA approved, sterile-filtered, suitable for cell culture

description

USA origin, Dialyzed by ultrafiltration against 0.15 M NaCl, sterile-filtered, suitable for cell culture

description

USA origin, suitable for cell culture

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

origin

USA origin

origin

Australia origin

origin

USA origin

origin

USA origin

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

biological source

bovine fetus

biological source

bovine fetus

biological source

bovine fetus

biological source

bovine fetus

composition

hemoglobin, ≤20 mg/dL

composition

Hemoglobin, ≤25 mg/dL

composition

Hemoglobin, ≤20 mg/dL , Bovine IgG, ≤1 mg/mL

composition

hemoglobin, ≤20 mg/dL

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


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Skladovací třída

10 - Combustible liquids

Co se děje?

WGK 3

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

PPE (osobní ochranné prostředky)

Eyeshields, Gloves



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    1 answer
    1. Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

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