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32670

4′,6-Diamidino-2-phenylindole dihydrochloride

suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

Synonyma:

2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride

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Vybrat velikost

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Vám/SKUDostupnostCena
5 mg
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
3 070,00 Kč
25 mg
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10 700,00 Kč

O této položce

Empirický vzorec (Hillův zápis):
C16H15N5 · 2HCl
Číslo CAS:
28718-90-3
Molekulová hmotnost:
350.25
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
57648699
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:
MFCD00012681
eCl@ss:
32150313

3 070,00 Kč

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product line

BioReagent

Quality Segment

assay

≥95.0% (HPLC)

form

powder

solubility

DMF: soluble, H2O: soluble

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA

suitability

suitable for fluorescence

storage temp.

room temp

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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Piktogramy

Exclamation mark
GHS07

Výstražné slovo

Warning

Kódy nebezpečí

H315,H317,H335

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

Cílové orgány

Respiratory system

Skladovací třída

11 - Combustible Solids

WGK

WGK 3

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

PPE (osobní ochranné prostředky)

Eyeshields, Gloves, type N95 (US)

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    Osvědčení o analýze (COA)

    Lot/Batch Number
    1337212
    BCCF5587
    BCBS7582V
    BCBZ8675
    BCBB8312V

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    Questions

    1–5 of 5 Questions  

    1. How can I store solutions of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?

      1 answer

      1. At 1 mg/mL, the solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored frozen at -20°C

        Helpful?

    2. What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear stain for cultured cells?

      1 answer

      1. Use DAPI at 0.1 microgram per mL in phosphate buffered saline (PBS) to incubate with monolayer cultured cells for 15-30 minutes at 30°C.  Immediately examine with a fluorescence microscope.

        Helpful?

    3. What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear counterstain for tissue sections?

      1 answer

      1. Following fluorescent staining of the cells, DAPI is added to the mounting media at 2 μg/ml.

        Helpful?

    4. What is the Department of Transportation shipping information for this product?

      1 answer

      1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

        Helpful?

    5. How can I make a solution of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?

      1 answer

      1. DAPI is soluble in water (10 mg/mL) with heat or sonication as necessary. It is insoluble in PBS even at 1 mg/ml.

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